The impact of chronic musculoskeletal pain on male reproductive hormones and sperm quality

A simple, precise, and accurate HPLC method has been developed and validated for assay of combined dosage form of Paracetamol and Thiocolchicoside in commercial pharmaceutical dosage form. Reversed-Phase liquid chromatographic analysis was performed on a BDS hypersil C18, 250mm × 4.6mm, 5µ(particle size), Thermo scientific column using Potassium Dihydrogen phosphate: Methanol (40:60, v/v) as eluent. The flow rate of the mobile phase was adjusted to 1.0 ml/min and the injection volume was 20 µl. Detection performed at 247nm. The retention time of Paracetamol and Thiocolchicoside were found to be 3.27 and 5.50 respectively. The method was validated for linearity, precision, accuracy, robustness. Response was a linear function of drug concentration in the range with 250-750 µg/ml for paracetamol and 1-3µg/ml for thiocolchicoside. Intraday and Interday precision were determined. Accuracy of Paracetamol and thiocolchicoside was found between 99-100%.All analytical validation parameters were determined by following the ICH guidelines and its limit. The developed method proclaimed to be precise and robust for the estimation of Paracetamol and