Robert P Goldman, Robert Moseley, Nicholas Roehner, Breschine Cummins, Justin D Vrana, Katie J Clowers, Daniel Bryce, Jacob Beal, Matthew DeHaven, Joshua Nowak, Trissha Higa, Vanessa Biggers, Peter Lee, Jeremy P Hunt, Lorraine Mosqueda, Steven B Haase, Mark Weston, George Zheng, Anastasia Deckard, Shweta Gopaulakrishnan, Joseph F Stubbs, Niall I Gaffney, Matthew W Vaughn, Narendra Maheshri, Ekaterina Mikhalev, Bryan Bartley, Richard Markeloff, Tom Mitchell, Tramy Nguyen, Daniel Sumorok, Nicholas Walczak, Chris Myers, Zach Zundel, Benjamin Hatch, James Scholz, John Colonna-Romano
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Highly-automated, high-throughput replication of yeast-based logic circuit design assessments.
We describe an experimental campaign that replicated the performance assessment of logic gates engineered into cells of Saccharomyces cerevisiae by Gander et al. Our experimental campaign used a novel high-throughput experimentation framework developed under Defense Advanced Research Projects Agency's Synergistic Discovery and Design program: a remote robotic lab at Strateos executed a parameterized experimental protocol. Using this protocol and robotic execution, we generated two orders of magnitude more flow cytometry data than the original experiments. We discuss our results, which largely, but not completely, agree with the original report and make some remarks about lessons learned. Graphical Abstract.