LANCL2的分子克隆、表征和组织特异性表达,LANCL2是lancl样蛋白家族的新成员

H. Mayer, Martina Pongratz, R. Prohaska
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引用次数: 19

摘要

我们鉴定并鉴定了人类LANCL2的cDNA编码,LANCL2是真核生物lancl样蛋白家族的一个新成员,与细菌镧硫氨酸合成酶组分C (LanC)有关。合成的核苷酸序列编码区为1353 bp, 5′-UTR为186 bp, 3′-UTR为2421 bp。与人类LANCL1同源序列相比,推断出的450个氨基酸序列同源性为57.9%(相似度为74.7%)。与LANCL1相比,LANCL2中发现了一个独特的ATP/ gtp结合位点基序a。Northern blot分析显示,在大脑中存在两个主要转录本,大小分别为4.7 kb和4.1 kb,在睾丸中存在一个主要的1.8 kb转录本。因此,表达阵列分析显示这些组织中有突出的信号。由于LANCL2与LanC的结构相似,我们推测LANCL2可能作为肽修饰复合物的组成部分发挥作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular Cloning, Characterization, and Tissue-Specific Expression of Human LANCL2, a Novel Member of the LanC-Like Protein Family
We identified and characterized the cDNA coding for human LANCL2, a new member of the eukaryotic LanC-like protein family which is related to the bacterial lanthionine synthetase components C (LanC). The composite nucleotide sequence revealed a coding region of 1353 bp, a 5′-UTR of 186 bp and a 3'-UTR of 2421 bp. The deduced sequence of 450 amino acids showed 57.9 % identity (74.7 % similarity) when compared with the human LANCL1 homologue. In contrast to LANCL1, a unique ATP/GTP-binding site motif A was found in LANCL2. Northern blot analysis revealed the presence of two major transcripts in the brain, 4.7 kb and 4.1 kb in size, and a major 1.8 kb transcript in testis. Accordingly, expression array analysis showed prominent signals in these tissues. Because of the structural similarity to LanC, we postulate that LANCL2 may play a role as a component of a pep-tide-modifying complex.
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