Green extraction using goat urine as menstruum and evaluation for in vitro antimycobacterial activity of Curcuma zedoaria and Curcuma caesia rhizomes collected from Assam

Background: In Indian traditional system of medicine, goat urine is believed to have therapeutic value and is also reported its use in the treatment of tuberculosis (TB). On the basis of reported traditional uses for the treatment of TB and/or leprosy, Curcuma caesia and Curcuma zedoaria rhizomes were selected. Aim: It was aimed to study the antimycobacterial activity of goat urine and extracts of the rhizome of the two plants obtained using goat urine as menstruum. Materials and Methods: The rhizomes were amassed from in and around Dibrugarh. The clean sliced rhizomes were dried at room temperature. The dried rhizomes of both the plant species were extracted using raw and photoactivated goat urine as menstruum by maceration process. In vitro antimycobacterial activity of the rhizome extracts was carried out by disc diffusion method. Results and Discussion: Crude photoactivated goat urine extracts of both the plants C. caesia (paGuCc) and C. zedoaria were found to have higher antimycobacterial activity against Mycobacterium smegmatis than that of raw goat urine extracts of both the plants C. caesia and C. zedoaria. Among all paGUCc extracts were found to exhibit highest antimycobacterial activity. Conclusion: The extracts obtained using photoactivated goat urine showed higher activity than the extracts obtained using raw goat urine. Goat urine also exhibited antimycobacterial activity, but not as much as the extracts. Thus, it is proved that the extracts and goat urine have antimycobacterial activity and extracting with goat urine and thus have improved activity.