{"title":"[β -榄香烯衍生物(ET)诱导NB4细胞凋亡的机制研究]。","authors":"Yu Zhiying","doi":"10.4268/CJCMM20101023","DOIUrl":null,"url":null,"abstract":"OBJECTIVE: beta-Elemene is an active component of herb medicine Curcuma wenyujin, N-(beta-elemene-13-yl)tryptophan (ET) was synthesized to increase the activity. The antiproliferative and apoptotic effects of ET were investigated with NB4 cell. METHOD: The mechanism of ET were studied with the technology of cell culture in vitro using MTT assay, flow cytometry and Western blot analysis. RESULT: ET inhibited cell growth and induced apoptosis in NB4 cell at concentrations less than 40 micromol x L(-1). The apoptosis induction of ET in NB4 cell was associated with the generation of hydrogen peroxide (H2O2) and activation of caspase-3. Catalase pretreatment blocked ET-induced apoptosis in NB4 cells. CONCLUSION: ET induces apoptosis through generation of H2O2 in NB4 cell.","PeriodicalId":9835,"journal":{"name":"China Journal of Chinese Matera Medica","volume":"21 1","pages":"1324"},"PeriodicalIF":0.0000,"publicationDate":"2010-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"[Mechanism study of beta-elemene derivative (ET) in NB4 cell on apoptosis induction].\",\"authors\":\"Yu Zhiying\",\"doi\":\"10.4268/CJCMM20101023\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"OBJECTIVE: beta-Elemene is an active component of herb medicine Curcuma wenyujin, N-(beta-elemene-13-yl)tryptophan (ET) was synthesized to increase the activity. The antiproliferative and apoptotic effects of ET were investigated with NB4 cell. METHOD: The mechanism of ET were studied with the technology of cell culture in vitro using MTT assay, flow cytometry and Western blot analysis. RESULT: ET inhibited cell growth and induced apoptosis in NB4 cell at concentrations less than 40 micromol x L(-1). The apoptosis induction of ET in NB4 cell was associated with the generation of hydrogen peroxide (H2O2) and activation of caspase-3. Catalase pretreatment blocked ET-induced apoptosis in NB4 cells. CONCLUSION: ET induces apoptosis through generation of H2O2 in NB4 cell.\",\"PeriodicalId\":9835,\"journal\":{\"name\":\"China Journal of Chinese Matera Medica\",\"volume\":\"21 1\",\"pages\":\"1324\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2010-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"China Journal of Chinese Matera Medica\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4268/CJCMM20101023\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"China Journal of Chinese Matera Medica","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4268/CJCMM20101023","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[Mechanism study of beta-elemene derivative (ET) in NB4 cell on apoptosis induction].
OBJECTIVE: beta-Elemene is an active component of herb medicine Curcuma wenyujin, N-(beta-elemene-13-yl)tryptophan (ET) was synthesized to increase the activity. The antiproliferative and apoptotic effects of ET were investigated with NB4 cell. METHOD: The mechanism of ET were studied with the technology of cell culture in vitro using MTT assay, flow cytometry and Western blot analysis. RESULT: ET inhibited cell growth and induced apoptosis in NB4 cell at concentrations less than 40 micromol x L(-1). The apoptosis induction of ET in NB4 cell was associated with the generation of hydrogen peroxide (H2O2) and activation of caspase-3. Catalase pretreatment blocked ET-induced apoptosis in NB4 cells. CONCLUSION: ET induces apoptosis through generation of H2O2 in NB4 cell.
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