不同pH值对公猪精子运动参数的影响

IF 0.7 4区 农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Cindy Rivas, M. E. Ayala, Andrés Aragón
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引用次数: 0

摘要

这项工作的目的是确定pH值对运动精子亚群结构的影响,使用在Androstar Plus扩展剂中稀释的商业剂量的野猪精液。我们将稀释后的精液样本的pH值调整为7.4、7.6、7.8和8.0,并使用开源计算机辅助精子分析系统评估运动参数。我们评估了单个精子的运动学参数数据集,以了解pH值对精子总体平均值和亚种群的影响。为了识别这些亚种群,我们通过主成分分析和层次聚类来降低数据集的维数。然后,我们将整个数据集和亚种群中的运动学参数的结果与相应控制的平均值(即基础pH值)进行比较。我们发现,增加pH值并不会影响精子的运动比例,但会影响它们运动的方式,使它们的运动更线性。在pH值高于7.1时,精子的进行性活力均较对照组增加,而在pH值为7.8时,精子的低活力比例增加。我们区分了三个精子亚群。在pH值为7.8和8.0时,亚群1的精子比例增加,且精子速度低,线性值高。相比之下,亚种群3的精子数量相对较少,但曲线速度相对较高。在这项研究中,我们证明了猪精子亚群的存在,它们对pH值的变化有不同的反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of various pH levels on the sperm kinematic parameters of boars
The objective of this work was to identify the effects of pH on the structure of kinematic sperm subpopulations using commercial doses of boar semen diluted in Androstar Plus extender. We adjusted the pH value of the diluted semen samples to 7.4, 7.6, 7.8, and 8.0, and evaluated motility parameters using an open-source computer-assisted sperm analysis system. We evaluated the dataset of the kinematic parameters of the individual sperm for the effects of pH on the average values overall and on the subpopulations of sperm. To identify these subpopulations, we reduced the dimensionality of the dataset through principal component analysis followed by hierarchical clustering. We then compared the results for the kinematic parameters across the dataset and in the subpopulations with the average of the corresponding control (i.e., the basal pH value). We found that increasing the pH did not affect the proportion of motile sperm but did affect the manner in which they moved, rendering their movement more linear. The progressive motility increased relative to the control at all pH values above 7.1, while the proportion of sperm with low motility increased at pH 7.8. We distinguished three sperm subpopulations. The proportion of sperm in Subpopulation 1 increased at pH values of 7.8 and 8.0, with the sperm being characterized by low velocity and high values for linearity. The sperm in Subpopulation 3, by contrast, were relatively few but showed relatively high curvilinear velocity. In this study, we demonstrated the presence of subpopulations of boar sperm that responded differentially to changes in pH.
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来源期刊
South African Journal of Animal Science
South African Journal of Animal Science 农林科学-奶制品与动物科学
CiteScore
1.50
自引率
0.00%
发文量
39
审稿时长
>36 weeks
期刊介绍: The South African Journal of Animal Science is an open access, peer-reviewed journal for publication of original scientific articles and reviews in the field of animal science. The journal publishes reports of research dealing with production of farmed animal species (cattle, sheep, goats, pigs, horses, poultry and ostriches), as well as pertinent aspects of research on aquatic and wildlife species. Disciplines covered nutrition, genetics, physiology, and production systems. Systematic research on animal products, behaviour, and welfare are also invited. Rigorous testing of well-specified hypotheses is expected.
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