V. Semenov, V. V. Pabiarzhyn, E. Pashinskaya, S. Egorov, V. Gorbachev, M. Kosova, A. Kabanava, N. A. Averchankava
{"title":"开发利用荧光PCR检测birc5和her-2 / neu基因在实验动物中的表达的测试系统，并对其有效性进行评估","authors":"V. Semenov, V. V. Pabiarzhyn, E. Pashinskaya, S. Egorov, V. Gorbachev, M. Kosova, A. Kabanava, N. A. Averchankava","doi":"10.22263/2312-4156.2021.2.65","DOIUrl":null,"url":null,"abstract":"Objectives. To develop primers and probes for detecting the expression of the BIRC5 and HER-2/neu genes and to optimize the reactions with the subsequent creation of PCR test systems with a fluorescent detection method. Material and methods. Achieving this goal included 5 stages, as a result of which two test systems were developed to determine the expression of the BIRC5 and HER-2/neu genes by real-time PCR in the experiment. Results. Two test systems for determining the expression of the BIRC5 and HER-2/neu genes in laboratory animals by means of PCR with fluorescence detection have been developed, that are highly sensitive and effective. Conclusions. The developed test system can be used for determining changes in the expression of the given genes during experiments of biological, veterinary and medical profiles.","PeriodicalId":23571,"journal":{"name":"Vestnik of Vitebsk State Medical University","volume":"39 1","pages":"65-70"},"PeriodicalIF":0.0000,"publicationDate":"2021-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"THE DEVELOPMENT OF TEST SYSTEMS FOR DETERMINING THE EXPRESSION OF THE BIRC5 AND HER-2/NEU GENES IN LABORATORY ANIMALS USING PCR WITH FLUORESCENCE DETECTION AND EVALUATION OF THEIR EFFECTIVENESS\",\"authors\":\"V. Semenov, V. V. Pabiarzhyn, E. Pashinskaya, S. Egorov, V. Gorbachev, M. Kosova, A. Kabanava, N. A. Averchankava\",\"doi\":\"10.22263/2312-4156.2021.2.65\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objectives. To develop primers and probes for detecting the expression of the BIRC5 and HER-2/neu genes and to optimize the reactions with the subsequent creation of PCR test systems with a fluorescent detection method. Material and methods. Achieving this goal included 5 stages, as a result of which two test systems were developed to determine the expression of the BIRC5 and HER-2/neu genes by real-time PCR in the experiment. Results. Two test systems for determining the expression of the BIRC5 and HER-2/neu genes in laboratory animals by means of PCR with fluorescence detection have been developed, that are highly sensitive and effective. Conclusions. The developed test system can be used for determining changes in the expression of the given genes during experiments of biological, veterinary and medical profiles.\",\"PeriodicalId\":23571,\"journal\":{\"name\":\"Vestnik of Vitebsk State Medical University\",\"volume\":\"39 1\",\"pages\":\"65-70\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-04-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Vestnik of Vitebsk State Medical University\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.22263/2312-4156.2021.2.65\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Vestnik of Vitebsk State Medical University","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22263/2312-4156.2021.2.65","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
THE DEVELOPMENT OF TEST SYSTEMS FOR DETERMINING THE EXPRESSION OF THE BIRC5 AND HER-2/NEU GENES IN LABORATORY ANIMALS USING PCR WITH FLUORESCENCE DETECTION AND EVALUATION OF THEIR EFFECTIVENESS
Objectives. To develop primers and probes for detecting the expression of the BIRC5 and HER-2/neu genes and to optimize the reactions with the subsequent creation of PCR test systems with a fluorescent detection method. Material and methods. Achieving this goal included 5 stages, as a result of which two test systems were developed to determine the expression of the BIRC5 and HER-2/neu genes by real-time PCR in the experiment. Results. Two test systems for determining the expression of the BIRC5 and HER-2/neu genes in laboratory animals by means of PCR with fluorescence detection have been developed, that are highly sensitive and effective. Conclusions. The developed test system can be used for determining changes in the expression of the given genes during experiments of biological, veterinary and medical profiles.
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