不同形式茶提取物(Camellia sinensis)对龋病病原菌的抑菌效果

Q3 Pharmacology, Toxicology and Pharmaceutics
V. Shivakumar, A. Tegginamani, N. Zain, Avita Rath, A. Zamzuri
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引用次数: 0

摘要

简介:龋齿是普通人群中一种常见的慢性感染性疾病。变形链球菌和嗜酸乳杆菌在引起龋齿中起重要作用。人们已经尝试了各种抗菌剂来预防这些微生物。从植物中提取的传统草药已被用作治疗方法。其中,茶叶提取物具有良好的抗感染活性。目的:研究不同类型茶提取物对致龋微生物的抑菌活性。方法:在体外实验研究中培养变形链球菌和嗜酸乳杆菌,并在各自的琼脂中维持。以日本绿茶、中国乌龙茶和沙巴红茶为原料,分别制备了250 mg/ml水溶液、250 mg/ml乙醇水溶液和250 mg/ml糖水溶液。采用Kirby-Bauer圆盘扩散法评价茶提取物的抑菌活性。阳性对照组和阴性对照组分别为0.2%氯己定和1% DMSO。37℃24小时孵育后,以毫米为单位测定抑制区。结果:3种不同浓度的日本绿茶对变形链球菌的平均抑制面积分别为18.33 mm、27.70 mm和18.96 mm, p<0.001。而氯己定对变形链球菌和嗜酸乳杆菌的影响分别为27.53 mm和19.39 mm。结论:日本绿茶在乙醇浓度下的抑菌活性优于其他浓度和其他两种茶。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antimicrobial Efficiency of Different forms of Tea Extract (Camellia sinensis) against Cariogenic Pathogens
Introduction: Dental caries is a frequent chronic infectious condition in the general population. Streptococcus mutans and Lactobacillus acidophilus play a significant part in the cause of dental caries. Various antimicrobials have been tried to prevent these microorganisms. Traditional herbal medicine extracted from plants has been employed as a remedy. Among them, tea leaf extract showed promising antimicrobial properties against infections. Aims: The current study was designed to assess the antibacterial activity of various types of tea extract against cariogenic microorganisms. Methods: S. mutans and L. acidophilus were grown in this in-vitro experimental study and maintained in their respective agars. Three different concentrations of 250 mg/ml aqueous, 250 mg/ml ethanolic, and 250 mg/ml aqueous with sugar solutions were prepared from Japanese green tea, Oolong Chinese tea, and Sabah black tea. Kirby-Bauer disc diffusion method was used to assess the antibacterial activity of tea extracts. As positive and negative control groups, 0.2% chlorhexidine and 1% DMSO were employed. The zone of inhibition was determined in millimetres following a 24-hour incubation period at 37 °C. Results: Japanese green tea at its three different concentrations exhibited significantly higher mean zones of inhibition of 18.33 mm, 27.70 mm, and 18.96 mm, respectively, against S. mutans at p<0.001 compared to L. acidophilus. In contrast, chlorhexidine showed 27.53 mm and 19.39 mm for S. mutans and L. acidophilus, respectively. Conclusion: Japanese green tea demonstrated superior antibacterial activity at its ethanolic concentration compared to other concentrations and the other two types of tea.
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来源期刊
Biomedical and Pharmacology Journal
Biomedical and Pharmacology Journal Pharmacology, Toxicology and Pharmaceutics-Pharmacology
CiteScore
1.20
自引率
0.00%
发文量
189
期刊介绍: Biomedical and Pharmacology Journal (BPJ) is an International Peer Reviewed Research Journal in English language whose frequency is quarterly. The journal seeks to promote research, exchange of scientific information, consideration of regulatory mechanisms that affect drug development and utilization, and medical education. BPJ take much care in making your article published without much delay with your kind cooperation and support. Research papers, review articles, short communications, news are welcomed provided they demonstrate new findings of relevance to the field as a whole. All articles will be peer-reviewed and will find a place in Biomedical and Pharmacology Journal based on the merit and innovativeness of the research work. BPJ hopes that Researchers, Research scholars, Academician, Industrialists etc. would make use of this journal for the development of science and technology. Topics of interest include, but are not limited to: Biochemistry Genetics Microbiology and virology Molecular, cellular and cancer biology Neurosciences Pharmacology Drug Discovery Cardiovascular Pharmacology Neuropharmacology Molecular & Cellular Mechanisms Immunology & Inflammation Pharmacy.
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