每周尿有机酸生物学变异

R. Lord, C. Nelson-Dooley, B. C. Morris, J. Bralley
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引用次数: 2

摘要

使用LC-MS/MS方法改进了样品制备,提高了尿液中40种或更多有机酸的测量通量。为了评估可能归因于营养不足或其他代谢紊乱的异常的重要性,需要确定正常生理反应导致的每周结果变化。本研究确定了22名健康成人8周的夜间尿液样本中37种有机酸、马尿酸、d -阿拉伯糖醇和8-羟基-2 '脱氧鸟苷的生物学变异性。对于40种分析物,CVb值从12.3到74.3不等。14个分析物的CVb值小于30,另外19个分析物的CVb值小于50。多种分析物显示出随浓度增加的变异性,这可能是大多数中间代谢物的特征。CVb与浓度的线性回归线斜率被制成表格,以帮助使用该信息。这40种分析物在疾病风险标记物(如血清脂蛋白胆固醇浓度、癌症标记物和甲状腺激素)范围内显示出生物学变异性。一次测量代表真实平均浓度的可能性随分析物和发现的水平而变化。这里报告的数据证明了在报告的分析条件下进行的尿有机酸谱分析结果的可靠性。[J] .中华医学杂志,2012;5(3):148-156。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Weekly Biological Variability of Urinary Organic Acids
Use of LC-MS/MS methods has improved sample preparation and increased throughput for the measurement of 40 or more organic acids in urine. In order to assess the significance of abnormalities that might be attributed to nutritional inadequacies or other metabolic disturbances, the week-to-week variation of results due to normal physiological responses needs to be established. This study determined the biological variability for 37 organic acids plus hippuric acid, D-arabinitol and 8-hydroxy-2’deoxyguanosine in overnight urine specimens from eight weekly samples submitted by 22 healthy adults. For the 40 analytes, CVb values varied from 12.3 to 74.3. Fourteen of the analytes had CVb values less than 30 and another 19 of them were less than 50. Multiple analytes displayed the property of increasing variability with concentration that may be characteristic of most intermediary metabolites. Linear regression line slopes for CVb vs. concentration were tabulated to assist the use of this information. The 40 analytes display biological variability in the range of disease risk markers such as serum lipoprotein cholesterol concentrations, cancer markers and thyroid hormones. The likelihood of a single measurement being representative of the true mean concentration varies with the analyte and the level found. Data reported here demonstrate reliability of results of urinary organic acid profiling performed under the reported analytical conditions. [N A J Med Sci. 2012;5(3): 148-156.]
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