{"title":"荆芥原生质体愈伤组织再生研究。","authors":"Jörg Jörgensen, Horst Binding","doi":"10.1016/S0044-328X(84)80044-6","DOIUrl":null,"url":null,"abstract":"<div><p>Protoplasts isolated from shoot tips of <em>Sorbus aucuparia</em> L. were cultivated in original and modified V-KM media at densities of 10<sup>5</sup> per ml, a temperature of 25±1°C, and 800 lx of cool white fluorescent light. Within six days of culture, 3% of the protoplasts showed mitotic activity. After 30 days, the cell clusters were transferred to medium B5C with 2.5 μM BA resulting in vigorous callus proliferation. So far, no organogenesis has been obtained even though various culture conditions have been applied.</p></div>","PeriodicalId":23797,"journal":{"name":"Zeitschrift für Pflanzenphysiologie","volume":"113 4","pages":"Pages 371-372"},"PeriodicalIF":0.0000,"publicationDate":"1984-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0044-328X(84)80044-6","citationCount":"10","resultStr":"{\"title\":\"Callus Regeneration with Protoplasts of Sorbus aucuparia L.\",\"authors\":\"Jörg Jörgensen, Horst Binding\",\"doi\":\"10.1016/S0044-328X(84)80044-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Protoplasts isolated from shoot tips of <em>Sorbus aucuparia</em> L. were cultivated in original and modified V-KM media at densities of 10<sup>5</sup> per ml, a temperature of 25±1°C, and 800 lx of cool white fluorescent light. Within six days of culture, 3% of the protoplasts showed mitotic activity. After 30 days, the cell clusters were transferred to medium B5C with 2.5 μM BA resulting in vigorous callus proliferation. So far, no organogenesis has been obtained even though various culture conditions have been applied.</p></div>\",\"PeriodicalId\":23797,\"journal\":{\"name\":\"Zeitschrift für Pflanzenphysiologie\",\"volume\":\"113 4\",\"pages\":\"Pages 371-372\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1984-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0044-328X(84)80044-6\",\"citationCount\":\"10\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zeitschrift für Pflanzenphysiologie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0044328X84800446\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zeitschrift für Pflanzenphysiologie","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0044328X84800446","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Callus Regeneration with Protoplasts of Sorbus aucuparia L.
Protoplasts isolated from shoot tips of Sorbus aucuparia L. were cultivated in original and modified V-KM media at densities of 105 per ml, a temperature of 25±1°C, and 800 lx of cool white fluorescent light. Within six days of culture, 3% of the protoplasts showed mitotic activity. After 30 days, the cell clusters were transferred to medium B5C with 2.5 μM BA resulting in vigorous callus proliferation. So far, no organogenesis has been obtained even though various culture conditions have been applied.
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