伊朗中部和东南部从流浪狗身上采集的蜱虫中检测出埃利希体

Q3 Veterinary

BULGARIAN JOURNAL OF VETERINARY MEDICINE Pub Date : 2022-01-01 DOI:10.15547/bjvm.2020-0128

H. Khovand, S. R. Nourollahi Fard, M. Khalili, M. Jajarmi, H. S. Hormozzaie

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引用次数: 0

摘要

埃立克体是人类和一些动物的埃立克体病的病原。血鼻头虫是犬埃利希体的主要媒介，狗、红狐和黄豺是该细菌的宿主。这种蜱在世界范围内分布，被认为是伊朗最常见的蜱类之一。本研究采用聚合酶链式反应(PCR)技术检测伊朗中部和东南部(伊斯法罕和扎博勒)流浪狗血种埃利希体，并对其流行情况进行评价。蜱虫样本是在2018年4月至6月期间从伊斯法罕和扎博尔的流浪狗身上收集的。采用商用试剂盒进行DNA提取。结果表明，伊斯法罕和扎布尔地区的埃利希菌库阳性率分别为21.42%和10%，而伊斯法罕和扎布尔地区的埃利希菌库阳性率均为15.21%。结果证实流浪狗血蜱中存在埃利希体，提示犬类及其蜱虫可能在该病流行病学中起重要作用。

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Detection of Ehrlichia spp. in ticks collected from stray dogs in Central and Southeastern Iran

Ehrlichia is an etiologic agent of ehrlichiosis in humans and some animals. Rhipicephalus sanguineus is the main vector of the Ehrlichia canis and dogs, red foxes and yellow jackals are reservoirs of the bacterium. This tick has a worldwide distribution and is regarded as one of the commonest species of ticks in Iran. This research aimed to detect Ehrlichia spp. in R. sanguineus isolated from stray dogs in Central and Southeast Iran (Isfahan and Zabol), by using polymerase chain reaction (PCR) and to evaluate the prevalence of the microorganism in these two areas. Tick samples were collected from stray dogs in Isfahan and Zabol between April and June of 2018. The DNA extraction was performed with commercial kits. PCR was done to determine the 336 bp fragment related to Ehrlichia spp. Overall, 15.21% of pools in both areas were positive for Ehrlichia, 21.42% and 10% of pools were from Isfahan and Zabol respectively. The results confirmed the presence of Ehrlichia spp. in R. sanguineus in stray dogs revealing that dogs and their ticks may have a significant role in the epidemiology of the disease.

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来源期刊

BULGARIAN JOURNAL OF VETERINARY MEDICINE Veterinary-Veterinary (all)

CiteScore

1.00

自引率

0.00%

发文量

审稿时长

15 weeks

期刊介绍： BJVM is a no-fee open-access scientific quarterly journal which covers topics related to both fundamental and applied aspects of veterinary medicine and to closely connected subjects with it. The journal publishes original papers, short communications and reviews.