{"title":"植物基因组编辑:CRISPR、碱基编辑、引体编辑等","authors":"Yujie Xie, Syed Inzimam Ul Haq, Xingyu Jiang, Dianfeng Zheng, Naijie Feng, Wenying Wang, Jin-Sheng He, Quan-Sheng Qiu","doi":"10.1002/glr2.12034","DOIUrl":null,"url":null,"abstract":"<p>The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system is a fast-growing, genome editing technology that has wide applications in identifying gene functions as well as improving agricultural production and crop breeding. Here, we summarized recent advances in the development and applications of genome editing technologies in plants. We briefly described CRISPR/Cas9 technology and examined the base and prime editing techniques that have been developed from CRISPR technology. Some new prime editing-derived techniques were assessed.</p>","PeriodicalId":100593,"journal":{"name":"Grassland Research","volume":"1 4","pages":"234-243"},"PeriodicalIF":0.0000,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/glr2.12034","citationCount":"2","resultStr":"{\"title\":\"Plant genome editing: CRISPR, base editing, prime editing, and beyond\",\"authors\":\"Yujie Xie, Syed Inzimam Ul Haq, Xingyu Jiang, Dianfeng Zheng, Naijie Feng, Wenying Wang, Jin-Sheng He, Quan-Sheng Qiu\",\"doi\":\"10.1002/glr2.12034\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system is a fast-growing, genome editing technology that has wide applications in identifying gene functions as well as improving agricultural production and crop breeding. Here, we summarized recent advances in the development and applications of genome editing technologies in plants. We briefly described CRISPR/Cas9 technology and examined the base and prime editing techniques that have been developed from CRISPR technology. Some new prime editing-derived techniques were assessed.</p>\",\"PeriodicalId\":100593,\"journal\":{\"name\":\"Grassland Research\",\"volume\":\"1 4\",\"pages\":\"234-243\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-12-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/glr2.12034\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Grassland Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/glr2.12034\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Grassland Research","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/glr2.12034","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
摘要
CRISPR /CRISPR相关蛋白9 (CRISPR -associated protein 9, Cas9)系统是一种快速发展的基因组编辑技术,在鉴定基因功能以及改善农业生产和作物育种方面有着广泛的应用。本文综述了近年来植物基因组编辑技术的发展和应用进展。我们简要介绍了CRISPR/Cas9技术,并研究了从CRISPR技术发展而来的碱基和引物编辑技术。评估了一些新的原始编辑衍生技术。
Plant genome editing: CRISPR, base editing, prime editing, and beyond
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system is a fast-growing, genome editing technology that has wide applications in identifying gene functions as well as improving agricultural production and crop breeding. Here, we summarized recent advances in the development and applications of genome editing technologies in plants. We briefly described CRISPR/Cas9 technology and examined the base and prime editing techniques that have been developed from CRISPR technology. Some new prime editing-derived techniques were assessed.