低强度激光照射下甲状腺肥大细胞年龄相关性形态功能变化的实验研究

D. A. Peshkova, E. Golovneva, D. R. Solyannikova
{"title":"低强度激光照射下甲状腺肥大细胞年龄相关性形态功能变化的实验研究","authors":"D. A. Peshkova, E. Golovneva, D. R. Solyannikova","doi":"10.37895/2071-8004-2022-26-3-4-68-74","DOIUrl":null,"url":null,"abstract":"Purpose: to study the dynamics of morphofunctional parameters of thyroid mast cells under low-intensity laser irradiation depending on age. Material and methods. The experiment was conducted on 96 white male laboratory rats of the Wistar breed. Animals were divided into four groups: 1 – control young, 2 – control old, 3 – young, low-intensity laser irradiation, 4 – old, low-intensity laser irradiation. A single laser irradiation session in continuous mode (wavelength – 970 nm, power – 1 W, exposure – 60 s) was made in the projection of thyroid gland. The animals were removed from the experiment 1 hour and 1, 3 and 7 days after exposure to laser light. Thyroid preparations were fixed in the formalin; histological sections were prepared and stained with toluidine blue; the morphometric analysis was performed to determine the number of thyroid mast cells and their varieties depending on the granular saturation level and degranulation degree.Results. The reaction of thyroid mast cells of experimental animals to laser exposure had some specific features: in young animals there was the increase in the number of cells and in their synthetic activity with the increase in granular saturation level and with the decrease in degranulation level which indicates an increased migration of cells into the thyroid gland and a pronounced adaptive reaction of the entire population of mast cells of the body. In old animals, exposure to laser light caused degranulation activity of thyroid mast cells with decrease in the level of granular saturation. No changes in the number of cells indicates that the reaction is limited within the organ contours.Conclusion. The results obtained expand our understanding of specific reactions of mast cells to laser exposure and confirm the need for individual correction of low-level laser therapy depending on age.","PeriodicalId":10008,"journal":{"name":"中国激光医学杂志","volume":"43 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Age-related morphofunctional changes of thyroid mast cells under low-intensity laser irradiation (an experimental study)\",\"authors\":\"D. A. Peshkova, E. Golovneva, D. R. Solyannikova\",\"doi\":\"10.37895/2071-8004-2022-26-3-4-68-74\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Purpose: to study the dynamics of morphofunctional parameters of thyroid mast cells under low-intensity laser irradiation depending on age. Material and methods. The experiment was conducted on 96 white male laboratory rats of the Wistar breed. Animals were divided into four groups: 1 – control young, 2 – control old, 3 – young, low-intensity laser irradiation, 4 – old, low-intensity laser irradiation. A single laser irradiation session in continuous mode (wavelength – 970 nm, power – 1 W, exposure – 60 s) was made in the projection of thyroid gland. The animals were removed from the experiment 1 hour and 1, 3 and 7 days after exposure to laser light. Thyroid preparations were fixed in the formalin; histological sections were prepared and stained with toluidine blue; the morphometric analysis was performed to determine the number of thyroid mast cells and their varieties depending on the granular saturation level and degranulation degree.Results. The reaction of thyroid mast cells of experimental animals to laser exposure had some specific features: in young animals there was the increase in the number of cells and in their synthetic activity with the increase in granular saturation level and with the decrease in degranulation level which indicates an increased migration of cells into the thyroid gland and a pronounced adaptive reaction of the entire population of mast cells of the body. In old animals, exposure to laser light caused degranulation activity of thyroid mast cells with decrease in the level of granular saturation. No changes in the number of cells indicates that the reaction is limited within the organ contours.Conclusion. The results obtained expand our understanding of specific reactions of mast cells to laser exposure and confirm the need for individual correction of low-level laser therapy depending on age.\",\"PeriodicalId\":10008,\"journal\":{\"name\":\"中国激光医学杂志\",\"volume\":\"43 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-03-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中国激光医学杂志\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.37895/2071-8004-2022-26-3-4-68-74\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中国激光医学杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.37895/2071-8004-2022-26-3-4-68-74","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

目的:研究低强度激光照射下甲状腺肥大细胞形态功能参数随年龄的变化规律。材料和方法。本实验以96只Wistar雄性白鼠为实验对象。实验动物分为4组:1组对照幼鼠,2组对照老年鼠,3组幼鼠,低强度激光照射,4组老年鼠,低强度激光照射。在连续模式下(波长- 970 nm,功率- 1 W,曝光- 60 s)进行单次激光照射甲状腺投影。分别于激光照射后1小时、1天、3天和7天离开实验。甲状腺制剂用福尔马林固定;制备组织切片,甲苯胺蓝染色;形态计量学分析确定甲状腺肥大细胞的数量及其种类取决于颗粒饱和度和脱粒程度。实验动物甲状腺肥大细胞对激光照射的反应有一些特殊的特点:在幼龄动物中,随着颗粒饱和度的增加和脱颗粒水平的降低,细胞数量和合成活性增加,这表明细胞向甲状腺的迁移增加,并且整个身体肥大细胞群体的适应性反应明显。在老年动物中,暴露于激光引起甲状腺肥大细胞的脱颗粒活性,颗粒饱和度降低。细胞数量没有变化,表明反应仅限于器官轮廓内。所获得的结果扩大了我们对肥大细胞对激光照射的特定反应的理解,并证实了根据年龄对低水平激光治疗进行个体校正的必要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Age-related morphofunctional changes of thyroid mast cells under low-intensity laser irradiation (an experimental study)
Purpose: to study the dynamics of morphofunctional parameters of thyroid mast cells under low-intensity laser irradiation depending on age. Material and methods. The experiment was conducted on 96 white male laboratory rats of the Wistar breed. Animals were divided into four groups: 1 – control young, 2 – control old, 3 – young, low-intensity laser irradiation, 4 – old, low-intensity laser irradiation. A single laser irradiation session in continuous mode (wavelength – 970 nm, power – 1 W, exposure – 60 s) was made in the projection of thyroid gland. The animals were removed from the experiment 1 hour and 1, 3 and 7 days after exposure to laser light. Thyroid preparations were fixed in the formalin; histological sections were prepared and stained with toluidine blue; the morphometric analysis was performed to determine the number of thyroid mast cells and their varieties depending on the granular saturation level and degranulation degree.Results. The reaction of thyroid mast cells of experimental animals to laser exposure had some specific features: in young animals there was the increase in the number of cells and in their synthetic activity with the increase in granular saturation level and with the decrease in degranulation level which indicates an increased migration of cells into the thyroid gland and a pronounced adaptive reaction of the entire population of mast cells of the body. In old animals, exposure to laser light caused degranulation activity of thyroid mast cells with decrease in the level of granular saturation. No changes in the number of cells indicates that the reaction is limited within the organ contours.Conclusion. The results obtained expand our understanding of specific reactions of mast cells to laser exposure and confirm the need for individual correction of low-level laser therapy depending on age.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
5650
期刊介绍: The main columns of "Chinese Journal of Laser Medicine & Surgery" include treatises, which report the latest research results in basic research and clinical trials in the field of laser medicine; comprehensive reviews of the latest research progress in laser medicine at home and abroad. In addition, there are short reports and excerpts from foreign journals, conference news and other columns. The journal has published a large number of papers on basic research on laser medicine and applied research in various clinical subjects, aiming to play a positive role in promoting the application of laser in medicine and improving the level of laser medicine research.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信