The extraction and identification of flavonoids from Nerium oleander L. in the east of Algeria - Constantine

In North Africa, Nerium oleander is quite common vegetation in the steppe zone. In Algeria also, its presence is quite common, especially on alluvial and stony soils. However, data on its phytochemical profiling are scanty. Therefore, the present study was designed to reveal phenolic diversity, identify some major flavonoid molecules, detect and elucidate the biological property of N. oleander leaves. Initially the extraction methods and strategies were optimized using various solvents and various phases were obtained. Subsequently, the phases obtained on various classes of phenolic molecules were confronted with analytical chromatography in the presence of an appropriate reagent and spectral analysis. Overall, five pure and native products namely kaemphferol, quercetin, luteolin, apigenin, quercetin 3OR were separated and purified.