Denise Guimarães Freire, Geraldo Lippel Sant'Anna Jr.
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引用次数: 7
摘要
葡萄糖淀粉酶(E.C. 3.2.1.3)共价固定在甲壳素颗粒(24-60目)上，平均活性为1000 U g−1。最佳温度和pH分别为60℃和3.6℃。这些值低于游离酶(pH为4·4，温度为65℃)的相应值。可溶性淀粉和水解木薯淀粉的Km (Michaelis常数)分别为1.25和3.94 g l - 1(游离酶)和8.6和7.8 g l - 1(固定化酶)。
Characterization of a glucoamylase immobilized on chitin
Glucoamylase (E.C. 3.2.1.3) covalently immobilized on chitin particles (24–60 mesh) showed an average activity of 1000 U g−1. Temperature and pH optima were 60°C and 3·6, respectively. These values were lower than the corresponding ones for the free enzyme (pH 4·4, temperature 65°C). The Km (Michaelis constant) values for soluble starch and hydrolyzed manioc starch were, respectively, 1·25 and 3·94 g litre−1 (free enzyme) and 8·6 and 7·8 g litre−1 (immobilized enzyme).
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