变稳定无性系雄蕊毛和花瓣自发和诱导体细胞粉色突变的分异模式

Marie Sanda-Kamigawara, M. Tomiyama, S. Ichikawa
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引用次数: 10

摘要

分析了花色(蓝/粉红)杂合的Tradescantia无性系雄蕊毛和花瓣中自发、辐射和ems诱导的体细胞粉色突变的分割模式。在室外培养的高变异克隆KU 20和受控环境条件下培养的稳定克隆KU 27和BNL 02分析了粉色的自发突变,在受控环境下培养的KU 27和BNL 02分析了粉色的诱导突变。在雄蕊毛的自发突变方面,三个无性系的单间质粉色突变事件与单端粉色突变事件之比均大于1,说明间质粉色突变事件的自发发生比末端粉色突变事件多。然而,在X射线、伽马射线或EMS处理后,这一比例在两个被检查的克隆中增加到约3,表明这些诱变剂诱导间质性粉红色突变事件比末端粉红色突变事件频繁得多。辐射和ems诱导的雄性雄蕊末端粉色突变体的分块模式的日变化与雄性雄蕊的发育模式吻合较好。在同一根头发中观察到的多个粉红色突变区比独立发生的频率要高得多,特别是在可变克隆中的自发突变和两个稳定克隆中的辐射诱导突变的情况下,这表明体细胞重组的参与。辐射和ems诱导的花瓣体细胞粉色突变的分割线也呈现出日变化,反映了花瓣发育的规律。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
SECTORING PATTERNS OF SPONTANEOUS AND INDUCED SOMATIC PINK MUTATIONS IN THE STAMEN HAIRS AND PETALS OF MUTABLE AND STABLE CLONES OF TRADESCANTIA
The sectoring patterns of spontaneous and radiation- and EMS-induced somatic pink mutations were analyzed in the stamen hairs and petals of Tradescantia clones heterozygous for flower color (blue/pink). Spontaneous pink mutations were analyzed using clone KU 20 (a highly mutable clone especially at lower temperature) grown outdoors and clones KU 27 and BNL 02 (stable clones) grown under controlled environmental conditions, while induced pink mutations were analyzed using clones KU 27 and BNL 02 grown under the controlled environments. As for spontaneous mutations in the stamen hairs, the ratio of the number of single interstitial pink mutant events against that of single terminal pink mutant events was somewhat larger than 1 in all the three clones examined, indicating that somewhat more interstitial pink mutant events occur spontaneously than terminal pink mutant events. After treatments with X rays, gamma rays or EMS, however, the ratio increased to about 3 in the two clones examined, showing much more frequent inductions of interstitial pink mutant events than terminal pink mutant events by these mutagens. The daily changes of the sectoring patterns of radiation- and EMS-induced terminal pink mutant events in the stamen hairs showed a good accordance with the pattern of the stamen-hair development. Multiple pink mutant sectors in the same hairs were observed at much higher frequencies than expected from independent occurrences, especially in cases of spontaneous mutations in the mutable clone and of radiation-induced mutations in the two stable clones, suggesting the involvement of somatic recombinations. The sectoring patterns of radiation- and EMS-induced somatic pink mutations in the petals also showed daily changes which reflected the pattern of the flower-petal development.
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