{"title":"间充质干细胞分泌组控制过敏性结膜炎炎症。","authors":"M. Baiula, S. Spampinato","doi":"10.21037/JES.2016.04.06","DOIUrl":null,"url":null,"abstract":"Su and colleagues (1) propose the use of culture medium from TNF-α pre-conditioned bone marrow-derived mesenchymal stem cells (MSC) as a novel therapeutic strategy for treating allergic conjunctivitis. MSC were exposed to TNF-α and conditioned medium was topically applied in the conjunctiva of mice with short ragweed pollen-induced experimental allergic conjunctivitis. Interestingly, ocular administration of TNF-α-conditioned medium reduced clinical signs and symptoms of allergic conjunctivitis. The anti-allergic effect of TNF-α-conditioned medium appeared to be due to the reduction of inflammatory cells infiltration and accumulation in the conjunctiva, inhibition of B cells, mast cells and histamine functions, through a COX-2-dependent mechanism. In fact, pre-treating MSC with COX-2 siRNA, the anti-allergic effects were abrogated. It seems that factors produced by MSC treated with TNF-α exert anti-allergic properties targeting simultaneously several key mediators of allergic reaction like B cells, mast cells, histamine and T helper 2 (Th2) cells.","PeriodicalId":12096,"journal":{"name":"眼科学报","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2016-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"Mesenchymal stem cell secretome to control inflammation in allergic conjunctivitis.\",\"authors\":\"M. Baiula, S. Spampinato\",\"doi\":\"10.21037/JES.2016.04.06\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Su and colleagues (1) propose the use of culture medium from TNF-α pre-conditioned bone marrow-derived mesenchymal stem cells (MSC) as a novel therapeutic strategy for treating allergic conjunctivitis. MSC were exposed to TNF-α and conditioned medium was topically applied in the conjunctiva of mice with short ragweed pollen-induced experimental allergic conjunctivitis. Interestingly, ocular administration of TNF-α-conditioned medium reduced clinical signs and symptoms of allergic conjunctivitis. The anti-allergic effect of TNF-α-conditioned medium appeared to be due to the reduction of inflammatory cells infiltration and accumulation in the conjunctiva, inhibition of B cells, mast cells and histamine functions, through a COX-2-dependent mechanism. In fact, pre-treating MSC with COX-2 siRNA, the anti-allergic effects were abrogated. It seems that factors produced by MSC treated with TNF-α exert anti-allergic properties targeting simultaneously several key mediators of allergic reaction like B cells, mast cells, histamine and T helper 2 (Th2) cells.\",\"PeriodicalId\":12096,\"journal\":{\"name\":\"眼科学报\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2016-04-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"眼科学报\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.21037/JES.2016.04.06\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"眼科学报","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.21037/JES.2016.04.06","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Mesenchymal stem cell secretome to control inflammation in allergic conjunctivitis.
Su and colleagues (1) propose the use of culture medium from TNF-α pre-conditioned bone marrow-derived mesenchymal stem cells (MSC) as a novel therapeutic strategy for treating allergic conjunctivitis. MSC were exposed to TNF-α and conditioned medium was topically applied in the conjunctiva of mice with short ragweed pollen-induced experimental allergic conjunctivitis. Interestingly, ocular administration of TNF-α-conditioned medium reduced clinical signs and symptoms of allergic conjunctivitis. The anti-allergic effect of TNF-α-conditioned medium appeared to be due to the reduction of inflammatory cells infiltration and accumulation in the conjunctiva, inhibition of B cells, mast cells and histamine functions, through a COX-2-dependent mechanism. In fact, pre-treating MSC with COX-2 siRNA, the anti-allergic effects were abrogated. It seems that factors produced by MSC treated with TNF-α exert anti-allergic properties targeting simultaneously several key mediators of allergic reaction like B cells, mast cells, histamine and T helper 2 (Th2) cells.