A. Fasya, Ariska Purwaning Tyas, F. Mubarokah, Rachmawati Ningsih, A. D. R. Madjid
{"title":"胶体直径和硅藻比的分离类固醇和脱氧核糖核酸红藻与湿色谱谱分析","authors":"A. Fasya, Ariska Purwaning Tyas, F. Mubarokah, Rachmawati Ningsih, A. D. R. Madjid","doi":"10.18860/al.v6i2.7015","DOIUrl":null,"url":null,"abstract":"Optimizing steroid and triterpenes isolation from red algae Eucheuma cottonii was done using column chromatography by varying of column diameter (1, 1.5 and 2 cm) and a ratio of sample : silica (1:50, 1:100, and 1:150). Active compound of E. cottonii was extracted by maceration with methanol. Then, the extract was hydrolyzed using HCl 2 N and partitioned with petroleum ether. The extract after partitioned was separated using wet column chromatography. The result of separation was monitored using analytical thin layer chromatography (Analytical TLC) and identified using FTIR. The best separation was aimed using diameter column 1 cm and a ratio of sample : silica 1:150. Based on FTIR analysis, the steroid from extract had a functional group –OH, –C–H, C=O, -C(CH3)2, and C-OH alcohol and the triterpenoid from extract had a functional group –OH, C–H, C=O, C=C, -C(CH3)2 and C-OH alcohol.Keywords: E. cottonii, column chromatography, steroid, triterpenoid, column size, sample : gel silica ratio Optimasi isolasi senyawa steroid dan triterpenoid dalam alga merah Euchema cottonii telah dilakukan menggunakan kromatografi kolom dengan variasi diameter kolom (1; 1,5; dan 2 cm) dan rasio sampel : silika (1:50; 1:100; dan 1:150). Ekstraksi senyawa aktif pada E. cottonii dilakukan dengan maserasi menggunakan pelarut metanol. Ekstrak pekat metanol dihidrolisis menggunakan HCl 2 N dan dipartisi dengan petroleum eter. Hasil pemisahan dimonitoring menggunakan KLTA dan diidentifikasi menggunakan FTIR. Hasil penelitian menunjukkan pemisahan kromatografi kolom terbaik diperoleh dengan variasi diameter 1 cm dan variasi rasion sampel : silika 1:150. Hasil analisis senyawa steroid memberikan informasi gugus –OH, –C–H, C=O, -C(CH3)2, dan C-OH alkohol, sedangkan senyawa triterpenoid memberikan informasi gugus –OH, –C–H, C=O, C=C, -C(CH3)2 dan C-OH alkohol.Kata Kunci: E. cottonii, kromatografi kolom, steroid, triterpenoid, ukuran kolom, rasio sampel : silika gel","PeriodicalId":31035,"journal":{"name":"Alchemy Journal of Chemistry","volume":"53 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2018-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Variasi Diameter Kolom dan Rasio Sampel-Silika pada Isolasi Steroid dan Triterpenoid Alga Merah Eucheuma cottonii dengan Kromatografi Kolom Basah\",\"authors\":\"A. Fasya, Ariska Purwaning Tyas, F. Mubarokah, Rachmawati Ningsih, A. D. R. 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Based on FTIR analysis, the steroid from extract had a functional group –OH, –C–H, C=O, -C(CH3)2, and C-OH alcohol and the triterpenoid from extract had a functional group –OH, C–H, C=O, C=C, -C(CH3)2 and C-OH alcohol.Keywords: E. cottonii, column chromatography, steroid, triterpenoid, column size, sample : gel silica ratio Optimasi isolasi senyawa steroid dan triterpenoid dalam alga merah Euchema cottonii telah dilakukan menggunakan kromatografi kolom dengan variasi diameter kolom (1; 1,5; dan 2 cm) dan rasio sampel : silika (1:50; 1:100; dan 1:150). Ekstraksi senyawa aktif pada E. cottonii dilakukan dengan maserasi menggunakan pelarut metanol. Ekstrak pekat metanol dihidrolisis menggunakan HCl 2 N dan dipartisi dengan petroleum eter. Hasil pemisahan dimonitoring menggunakan KLTA dan diidentifikasi menggunakan FTIR. Hasil penelitian menunjukkan pemisahan kromatografi kolom terbaik diperoleh dengan variasi diameter 1 cm dan variasi rasion sampel : silika 1:150. 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引用次数: 2
摘要
采用柱层析法，通过改变柱径(1、1.5和2 cm)和样品与二氧化硅的比例(1:50、1:100和1:150)，对赤藻真棉中甾体和三萜的分离进行优化。采用甲醇浸渍法提取棉草的活性成分。然后用盐酸水解提取液，用石油醚对提取液进行分馏。分离后的提取液采用湿柱色谱法进行分离。用分析薄层色谱法(TLC)监测分离结果，并用FTIR进行鉴定。柱径为1cm，样品与二氧化硅的比为1:150，分离效果最佳。FTIR分析表明，提取液中的甾体具有-OH、-C - h、C=O、-C(CH3)2和C-OH醇的官能团;提取液中的三萜具有-OH、-C - h、C=O、C=C、-C(CH3)2和C-OH醇的官能团。关键词:棉草，柱层析，甾体，三萜，柱尺寸，样品:凝胶-二氧化硅比;1、5;丹2厘米)丹拉西奥样品:硅胶(1:50;1:10 0;丹1:15)。【关键词】紫杉醇;紫杉醇;紫杉醇;甲醇二元化蒙古那坎盐酸二元化丹根石油计。哈西尔·派米沙汗监测孟古纳坎KLTA和鉴别孟古纳坎FTIR。Hasil penelitian menunjukkan pesmiahan kromatografi kolom terbaik diperoleh dengan variasi直径1 cm丹variasi样品:硅1:150。Hasil分析了senyawa甾类化合物-OH， -C - h, C=O， -C(CH3)2，和C-OH醇，sedangkan senyawa三萜化合物-OH， -C - h, C=O, C=C， -C(CH3)2和C-OH醇。卡塔昆奇:棉草，红草，甾体，三萜，乌库兰，硅胶
Variasi Diameter Kolom dan Rasio Sampel-Silika pada Isolasi Steroid dan Triterpenoid Alga Merah Eucheuma cottonii dengan Kromatografi Kolom Basah
Optimizing steroid and triterpenes isolation from red algae Eucheuma cottonii was done using column chromatography by varying of column diameter (1, 1.5 and 2 cm) and a ratio of sample : silica (1:50, 1:100, and 1:150). Active compound of E. cottonii was extracted by maceration with methanol. Then, the extract was hydrolyzed using HCl 2 N and partitioned with petroleum ether. The extract after partitioned was separated using wet column chromatography. The result of separation was monitored using analytical thin layer chromatography (Analytical TLC) and identified using FTIR. The best separation was aimed using diameter column 1 cm and a ratio of sample : silica 1:150. Based on FTIR analysis, the steroid from extract had a functional group –OH, –C–H, C=O, -C(CH3)2, and C-OH alcohol and the triterpenoid from extract had a functional group –OH, C–H, C=O, C=C, -C(CH3)2 and C-OH alcohol.Keywords: E. cottonii, column chromatography, steroid, triterpenoid, column size, sample : gel silica ratio Optimasi isolasi senyawa steroid dan triterpenoid dalam alga merah Euchema cottonii telah dilakukan menggunakan kromatografi kolom dengan variasi diameter kolom (1; 1,5; dan 2 cm) dan rasio sampel : silika (1:50; 1:100; dan 1:150). Ekstraksi senyawa aktif pada E. cottonii dilakukan dengan maserasi menggunakan pelarut metanol. Ekstrak pekat metanol dihidrolisis menggunakan HCl 2 N dan dipartisi dengan petroleum eter. Hasil pemisahan dimonitoring menggunakan KLTA dan diidentifikasi menggunakan FTIR. Hasil penelitian menunjukkan pemisahan kromatografi kolom terbaik diperoleh dengan variasi diameter 1 cm dan variasi rasion sampel : silika 1:150. Hasil analisis senyawa steroid memberikan informasi gugus –OH, –C–H, C=O, -C(CH3)2, dan C-OH alkohol, sedangkan senyawa triterpenoid memberikan informasi gugus –OH, –C–H, C=O, C=C, -C(CH3)2 dan C-OH alkohol.Kata Kunci: E. cottonii, kromatografi kolom, steroid, triterpenoid, ukuran kolom, rasio sampel : silika gel
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