Comparative Evaluation of Ultraviolet, Microwave and Antimicrobial Sterilization Techniques for Toothbrush Decontamination

Objective: This study aimed to study the antimicrobial efficacy of sanitization of toothbrushes using: Ultraviolet (UV) light sanitizer, domestic microwave oven (MO), 0.2% chlorhexidine gluconate (CHX) and to estimate the microbial contamination of toothbrushes when stored: inside the bathroom and outside the bathroom. Materials and Methods: The toothbrushes were divided into two groups: Group I (n=45) stored inside the bathroom and Group II (n=45) stored outside the bathroom. Group I and Group II was further sub divided into three groups and then subjected to different sanitization methods: Ultraviolet light sanitizer, domestic microwave oven, and 0.2% chlorhexidine gluconate. Results: Contamination is found to be less in the brushes kept outside the bathroom as compared to brushes kept inside the bathroom and Most effective method for sanitization of toothbrushes is microwave irradiation followed by UV rays and then chlorhexidine gluconate solution. Conclusion: Toothbrushes should always be stored outside the bathroom so that they are out of the aerosol range and proper storage & disinfection of the toothbrush is as important as brushing your teeth for a healthy and germ-free life.