{"title":"大鼠脑突触囊泡蛋白在肉毒杆菌神经毒素敏感和特异性检测中的应用","authors":"M. Singh, V. Chauhan, R. Dhaked","doi":"10.4172/2157-2526.S2-008","DOIUrl":null,"url":null,"abstract":"We propose here the application of synaptic vesicle proteins isolated from rat brain as a sole substrate for the specific endoproteinase activities of all seven serotypes of Botulinum Neurotoxin (BoNT/A to G). In this study, we used these proteins for evaluating endopeptidase and receptor binding activity for detecting BoNT/A by western blot and surface plasmon resonance with 6.25 pM and 0.22 fM limit of detection, respectively. Substrate and receptor present in the synaptic vesicle proteins are very robust and stable for more than 6 months to use in BoNT detection.","PeriodicalId":15179,"journal":{"name":"Journal of Bioterrorism and Biodefense","volume":"19 1","pages":"1-3"},"PeriodicalIF":0.0000,"publicationDate":"2012-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Applications of Rat Brain Synaptic Vesicle Proteins for Sensitive and Specific Detection of Botulinum Neurotoxins\",\"authors\":\"M. Singh, V. Chauhan, R. Dhaked\",\"doi\":\"10.4172/2157-2526.S2-008\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We propose here the application of synaptic vesicle proteins isolated from rat brain as a sole substrate for the specific endoproteinase activities of all seven serotypes of Botulinum Neurotoxin (BoNT/A to G). In this study, we used these proteins for evaluating endopeptidase and receptor binding activity for detecting BoNT/A by western blot and surface plasmon resonance with 6.25 pM and 0.22 fM limit of detection, respectively. Substrate and receptor present in the synaptic vesicle proteins are very robust and stable for more than 6 months to use in BoNT detection.\",\"PeriodicalId\":15179,\"journal\":{\"name\":\"Journal of Bioterrorism and Biodefense\",\"volume\":\"19 1\",\"pages\":\"1-3\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2012-05-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Bioterrorism and Biodefense\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4172/2157-2526.S2-008\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Bioterrorism and Biodefense","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4172/2157-2526.S2-008","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Applications of Rat Brain Synaptic Vesicle Proteins for Sensitive and Specific Detection of Botulinum Neurotoxins
We propose here the application of synaptic vesicle proteins isolated from rat brain as a sole substrate for the specific endoproteinase activities of all seven serotypes of Botulinum Neurotoxin (BoNT/A to G). In this study, we used these proteins for evaluating endopeptidase and receptor binding activity for detecting BoNT/A by western blot and surface plasmon resonance with 6.25 pM and 0.22 fM limit of detection, respectively. Substrate and receptor present in the synaptic vesicle proteins are very robust and stable for more than 6 months to use in BoNT detection.
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