形状各向异性磁性颗粒，用于高通量和高效率的功能大分子细胞内递送

2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS) Pub Date : 2015-06-21 DOI:10.1109/TRANSDUCERS.2015.7181064

M. Lin, Y. C. Wu, J. Lee, J. Zhou, M. Teitell, P. Chiou

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引用次数: 0

摘要

我们报道了一种新的基于形状各向异性磁颗粒(SAMP)对齐的细胞内递送系统，以高效率和高活力将功能分子递送到哺乳动物细胞中。广泛的分子(钙黄蛋白、葡聚糖3K、葡聚糖40K、GFP质粒、SiRNA和β-内酰胺酶)已成功递送到原代细胞中，并保留了功能。我们已经实现了将酶高效递送至原代人真皮成纤维细胞(NHDF)，将其他大分子(葡聚糖40K)高效递送至小鼠皮质神经元。

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Shape Anisotropic Magnetic Particles for high throughput and high efficiecy intracelluar delivery of functional macromolecules

We report a novel intracellular delivery system based on aligning Shape-Anisotropic Magnetic Particles (SAMP) to deliver functional molecules into mammalian cells with high efficiency and high viability. A broad range of molecules (calcein, dextran 3K, dextran 40K, GFP plasmid, SiRNA, and β-lactamase) have been successfully delivered into primary cells with preserved functions. We have achieved high efficiency delivery of enzyme into primary human dermal fibroblast (NHDF), and other large macromolecules (dextran 40K) into mouse cortical neurons.

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2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS)

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