稀土元素镧对盐胁迫下蔬菜生长及抗氧化系统的影响

Meihua Fan, Z. Liao, Li Chen, Ge Shi, Jian Xin Wang
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引用次数: 3

摘要

研究了稀土元素镧对盐胁迫下青菜生长和抗氧化系统的影响。青果种子在含50mg·L-1 NaCl和20、40、60、80、100、150mg·L-1镧的Hoagland营养液中培养。然后以50mg·L-1 NaCl、20mg·L-1镧为对照的Hoagland营养液培养。持续2周后,测定种子发芽率和超氧化物歧化酶(SOD)、过氧化物酶(POD)活性、丙二醛(MDA)。结果表明,盐胁迫对青菜种子萌发和幼苗生长有抑制作用,鲜重明显降低,幼苗矮化。低浓度LaCl3(20~80mg·L-1)能促进青菜种子萌发和幼苗生长,鲜重显著增加,苗高。盐胁迫显著降低了青菜幼苗叶绿素含量,添加适宜浓度的LaCl3 (20~100mg·L-1)可提高盐胁迫下青菜幼苗叶绿素含量,增强其光转化能力。而在20mg·L-1 LaCl3处理的hoagland营养液处理下,青菜幼苗叶绿素含量明显降低(P3 (40~80mg·L-1)能够缓解盐胁迫的压力,提高青菜幼苗可溶性蛋白含量。高浓度LaCl3(100~150mg·L-1)在盐胁迫下明显抑制其可溶性。果蔬幼苗的蛋白质产品。盐胁迫可刺激青菜幼苗诱导其抗氧化酶保护系统,使青菜幼苗抗氧化酶增加,MDA含量降低,盐胁迫下适宜浓度LaCl3(40 ~ 80mg·L-1)可促进抗氧化酶的产生,减轻盐胁迫对幼苗的伤害,进而降低MDA含量。但高水平的LaCl3 (100~150mg·L-1)可能对细胞产生毒性作用,导致抗氧化酶活性减弱,MDA含量升高。总之,盐胁迫(50mg·L-1)可抑制青果种子萌发和幼苗生长,诱导抗氧化酶出现,MDA含量升高。适宜浓度的LaCl3处理能显著提高青菜幼苗抗氧化酶活性,增强其对活性氧的清除能力,降低盐胁迫下的单氧化脂质,从而有效缓解盐胁迫伤害,提高青菜幼苗的耐盐性,促进青菜幼苗生长,适宜浓度为40~80mg·L-1。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of Rare Earth Elements Lanthanum on Growth and Antioxidation System of the Greengrocery under Salt Stress
The effects of rare earth elements lanthanum on growth and antioxidation system of the greengrocery under salt stress were investigated. The seed of greengrocery were cultureed in Hoagland nutrient solution with 50mg·L-1 NaCl and 20, 40, 60, 80, 100, 150mg·L-1 Lanthanum. Then the treatments cultured in Hoagland nutrient solution with 50mg·L-1 NaCl, 20mg·L-1 lanthanum as control. After continued for two weeks, seed germination rate and super oxide dismutase(SOD) and peroxidase ( POD) activities, malondialdehyde (MDA) were measured. The results showed that salt stress could inhibite seed germination and seedling growth of greengrocery, fresh weight marked reduced, dwarf seedlings. Low concentration LaCl3(20~80mg·L-1)could promote seed germination and seedling growth of greengrocery, fresh weight marked increased, high seedlings. Salt stress significantly reduced the chlorophyll content of and adding suitable concentration LaCl3 (20~100mg·L-1 )could improve of chlorophyll content of the greengrocery seedlings under salt stress and enhance its light transformation ability. But under hoagland nutrient solution with 20mg·L-1 LaCl3 processing, chlorophyll content of the greengrocery seedlings had marked reduced(P<;0.05 ). Salt stress can induce the generation of protein. The treaments with proper density LaCl3 (40~80mg·L-1) were able to alleviate the pressure of salt stress, and improve the soluble protein content of greengrocery seedling. High concentration LaCl3(100~150mg·L-1) under salt stress marked inhibited the the soluble. protein produce of greengrocery seedling. Salt stress could stimulate greengrocery seedlings induction their antioxidant enzymes protection system, make antioxidant enzymes of greengrocery seedling increase, MDA content reduced, Suitable concentration LaCl3(40-80mg·L-1) under salt stress can promote antioxidant enzymes relieve the damage of salt stress on seedling, then MDA content reduced. but high levels of LaCl3 (100~150mg·L-1)is likely to produce poisonous effect on cells leaded to antioxidant enzymes activity abate, MDA content increased. In brief, salt stress(50mg·L-1) could inhibited seed germination and seedling growth of greengrocery, and induction antioxidant enzymes emerge, MDA content increased. The treatment with optimum concentration of LaCl3 could significantly increase antioxidant enzyme activities of greengrocery seedlings, enhance its scavenging reactive oxygen species, reduce lipid per oxidation under salt stress, in order to effectively alleviate the salt stress injury and increase their salt tolerance, and promote greengrocery seedling growth, The optium concentration was 40~80mg·L-1.
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