{"title":"绿豆(Vigna radiata)中生菌根瘤菌的流行","authors":"P. K. Maan, S. Garcha, G. S. Walia","doi":"10.18805/LR-3884","DOIUrl":null,"url":null,"abstract":"The present work was undertaken to ascertain prevalence of bacteriocinogenic Rhizobium spp. in mungbean (Vigna radiata). Samples of rhizospheric soil and nodules were plated onto CRYEMA medium, selective for Rhizobium spp. From among the isolates obtained only four, designated as-N8, S1, S6, and S13 demonstrated bacteriocin production. Upto 104 AU/ml (Arbitrary Units/ml) of partially purified bacteriocin (PPB) was detected from N8 and S6. PPB obtained from N8 and S13 exhibited antagonism against indicator strain over a wide range of pH. All isolates of PPB were thermo-stable after heating to 90oC for 5 minutes. N8 exhibited 14.2mm zone of inhibition after heating to 50oC for 10 min. It decreased by 29.5% to 10mm (80oC/ 10min). S6 exhibited zone of inhibition of 11mm (50oC/10min) which decreased by 18.1% to 9mm (80oC/10min). Dual inoculation of nitrogen fixing Rhizobium with bacteriocin producing Rhizobium can ensure greater benefit than using nitrogen fixer alone.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":null,"pages":null},"PeriodicalIF":0.8000,"publicationDate":"2018-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"Prevalence of bacteriocinogenic Rhizobium spp. in mungbean (Vigna radiata)\",\"authors\":\"P. K. Maan, S. Garcha, G. S. Walia\",\"doi\":\"10.18805/LR-3884\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The present work was undertaken to ascertain prevalence of bacteriocinogenic Rhizobium spp. in mungbean (Vigna radiata). Samples of rhizospheric soil and nodules were plated onto CRYEMA medium, selective for Rhizobium spp. From among the isolates obtained only four, designated as-N8, S1, S6, and S13 demonstrated bacteriocin production. Upto 104 AU/ml (Arbitrary Units/ml) of partially purified bacteriocin (PPB) was detected from N8 and S6. PPB obtained from N8 and S13 exhibited antagonism against indicator strain over a wide range of pH. All isolates of PPB were thermo-stable after heating to 90oC for 5 minutes. N8 exhibited 14.2mm zone of inhibition after heating to 50oC for 10 min. It decreased by 29.5% to 10mm (80oC/ 10min). S6 exhibited zone of inhibition of 11mm (50oC/10min) which decreased by 18.1% to 9mm (80oC/10min). Dual inoculation of nitrogen fixing Rhizobium with bacteriocin producing Rhizobium can ensure greater benefit than using nitrogen fixer alone.\",\"PeriodicalId\":18181,\"journal\":{\"name\":\"Legume Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2018-05-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Legume Research\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.18805/LR-3884\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Legume Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.18805/LR-3884","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRONOMY","Score":null,"Total":0}
Prevalence of bacteriocinogenic Rhizobium spp. in mungbean (Vigna radiata)
The present work was undertaken to ascertain prevalence of bacteriocinogenic Rhizobium spp. in mungbean (Vigna radiata). Samples of rhizospheric soil and nodules were plated onto CRYEMA medium, selective for Rhizobium spp. From among the isolates obtained only four, designated as-N8, S1, S6, and S13 demonstrated bacteriocin production. Upto 104 AU/ml (Arbitrary Units/ml) of partially purified bacteriocin (PPB) was detected from N8 and S6. PPB obtained from N8 and S13 exhibited antagonism against indicator strain over a wide range of pH. All isolates of PPB were thermo-stable after heating to 90oC for 5 minutes. N8 exhibited 14.2mm zone of inhibition after heating to 50oC for 10 min. It decreased by 29.5% to 10mm (80oC/ 10min). S6 exhibited zone of inhibition of 11mm (50oC/10min) which decreased by 18.1% to 9mm (80oC/10min). Dual inoculation of nitrogen fixing Rhizobium with bacteriocin producing Rhizobium can ensure greater benefit than using nitrogen fixer alone.
期刊介绍:
Legume Research An International Journal. The Journal is an official publication of Agricultural Research Communication Centre. It is designed to bring out the original research articles on genetics, breeding, physiology, bacterial avtivity, production, quality, biochemistry and seeds of legumes crops. The objective of the journal is to serve as a forum for scientific community to publish their research findings on legumes ans to provide basis for new research. Journal is being scanned in the important indexing and abstracting services.