亚慢性铝中毒对鸡大脑神经细胞凋亡的影响:铝蓄积对鸡神经系统影响的初步研究

Hao Sun, Yan-fei Li, Chong-wei Hu, Y. She, Lingyu Gao
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引用次数: 1

摘要

采用固定体积、不同梯度三氯化铝(AlCl3)连续腹腔注射的方法,建立了鸡不同程度亚慢性铝中毒模型。采用琼脂糖凝胶电泳、AO/EB双荧光染色和流式细胞术检测大脑神经细胞凋亡情况。结果表明:(1)荧光显微镜下观察到典型的脑神经细胞凋亡和坏死,且各实验组的凋亡指数显著高于对照组(P<0.01),且随铝浓度的升高而升高;(2)大脑神经细胞数量在G0/G1期增加,在G2/M期和S期减少。细胞增殖指数降低,细胞凋亡率升高,与对照组相比差异极显著(P<0.01);(3)琼脂糖凝胶电泳显示了实验组鸡大脑神经细胞典型的DNA阶梯状结构。结果表明,铝能引起大脑细胞DNA损伤,抑制细胞增殖,诱导细胞凋亡。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of Sub-Chronic Aluminum Intoxication on Apoptosis of Cerebrum Neurocytes in Chickens: Preliminary Study on Effect of Aluminum Accumulation on Nervous System in Chickens
The model of different level's sub-chronic aluminum intoxication in chickens was established by continuous peritoneal injection of fixed volume and different gradient aluminum trichloride (AlCl3). The apoptosis of cerebrum neurocytes was detected by agarose gel electrophor-esis, AO/EB double fluorescent staining and flow cytometry. The results showed that: (1) The typical apoptosis and necrosis of cerebrum neurocytes were observed in the fluorescence microscope, and the apoptotic index in the experimental groups was significantly higher than that in the control groups (P<0.01), which increased along with increase of aluminum concentration; (2) The number of cerebrum neurocytes increased in G0/G1 phases, while decreased in G2/M and S phases. The cell proliferation index decreased and apoptosis rate increased, which were significantly different compared with the control ones (P<0.01); (3) The typical DNA Ladder-shaped of cerebrum neurocytes of chickens in experimental groups was displayed by agarose gel electropho- resis. The results indicated that aluminum can cause the DNA damage, inhibite the proliferation and induce the apoptosis of cerebrum cell.
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