L. Friedman, D. Gaines, R. Chi, Matthew C Smith, R. Braunberg, C. Thorpe
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引用次数: 4
摘要
通过测定食物中常见的黄曲霉毒素对年轻成熟大鼠肝脏和肝细胞的生化作用,评估了它们之间可能的相互作用。RNA合成是黄曲霉毒素B1作用的一个敏感的早期靶点,我们用[C]山茱萸酸或3 [H]尿苷作为底物来测定RNA合成。在第一项研究(由四个复制实验),肝片治疗黄曲霉毒素B1(0, 120, 240,和480 ng / ml孵化的存在和缺乏黄曲霉素B2 120 ng / ml。在第二项研究中,肝片治疗与山姆e浓度存在和缺乏黄曲霉毒素B1孵化的G1在120 ng / l(由四个复制experim树人)或240 ng / ml(由两个复制experim树人)。在第三项研究(单实验)中,分离的肝细胞分别用黄曲霉毒素B1 0、60、120、240和480 ng/ ml处理,黄曲霉毒素G1浓度分别为120 ng/ ml和不含黄曲霉毒素G1。
INTERACTION OF AFLATOXINS AS MEASURED BY THEIR BIOCHEMICAL ACTION ON RAT LIVER SLICES AND HEPATOCYTES
The possible interaction between pairs of aflatoxins com monly occurring together in foods was evaluated by determining their biochem ical actions on the liver and hepatocytes of young mature rats. RNA synthesis, a sensitive and 14 early target of the effects of aflatoxin B1, was m easured with [ C]orotic acid or 3 [ H]uridine used as substrates. In the first study (comprised of four replicate experiments), liver slices treated with aflatoxin B1 at 0, 120, 240, and 480 ng/ ml were incubated in the presence and absence of aflatoxin B2 at 120 ng/ ml. In the second study, liver slices were treated with the sam e concentrations of B1 incubated in the presence and absence of aflatoxin G1 at 120 ng/ m l (comprised of four replicate experim ents) or at 240 ng/ ml (comprised of two replicate experim ents). In the third study (single experim ent), isolated hepatocytes were treated with aflatoxin B1 at 0, 60, 120, 240, and 480 ng/ ml in the presence and absence of aflatoxin G1 at 120 ng/ m l. Leakage of lactic acid...