金针叶花、叶、茎己烷提取物α -葡萄糖苷酶抑制及抗氧化活性研究和阿魏(haussknechtii Wolff ex Rech)。

M. Zarei, Hero Ghafaryan
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引用次数: 2

摘要

背景:α-葡萄糖苷酶抑制可显著阻止糖尿病患者餐后葡萄糖摄取,有助于控制一些不良反应。因此,本研究的目的是测定金合欢和阿魏空中器官α-葡萄糖苷酶的抑制作用和抗氧化活性。方法:利用酶标仪在405 nm波长下研究不同浓度下不同器官己烷提取物的抑菌作用。采用DPPH和铁还原试验测定了各器官己烷提取物的抗氧化活性。结果:花提取物浓度为0.1 g/ml(100%抑制,IC50= 0.1 μg /ml)时,对haussknechtii的抑制活性最高;金针叶提取物浓度为1 g/ml(100%抑制,IC50= 10 μg /ml)和叶提取物浓度为100%抑制,IC50= 60 μg /ml时,对金针叶的抑制活性最高。金针叶提取物在0.001 g/ml浓度下表现为竞争-非竞争混合抑制模式,0.1 g/ml浓度下表现为非竞争抑制模式。结果表明,金针叶和金针叶的EC50分别为2.37和0.96 mg/ml。结论:金针叶花和金针叶花及叶器官的己烷提取物对α-葡萄糖苷酶活性、DPPH自由基清除能力和还原能力均有显著的抑制作用。为糖尿病患者餐后控制血糖的药用化合物的提取提供了良好的资源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Alfa-Glucosidase inhibitory and antioxidant activity of hexane extract of flowers, leave and stems of Haplophyllum acutifolium DC. and Ferula haussknechtii Wolff ex Rech.
Background:α-Glucosidase Inhibition can significantly prevent glucose uptake after meal, and helps in controlling of some adverse effects in diabetics. So determination of α-Glucosidase inhibitory effect and antioxidant activity of the Haplophyllum acutifolium and Ferula haussknechtii aerial organs was the aim of this study.Methods:Inhibitory effect of hexane extracts from different organs was investigated in several concentrations at 405 nm wavelength using a microplate reader. Antioxidant activity of hexane extracts of various organs was also measured using DPPH and iron reduction tests. Results;The highest inhibitory activity of F.haussknechtii was observed at the 0.1 g/ml concentration of flower extract (100% inhibition and IC50= 0.1 μg /ml) and the most inhibitory activity of H.acutifolium, was related to the 1 g/ml concentration of flower extract (100% inhibition and IC50 = 10 μg /ml) and leaf extract (100% inhibition and IC50 = 60 μg /ml). Extract of H.acutifolium flower and leaves showed Non-competitive inhibition pattern and F.haussknechtii flower showed mixed (Competitive -Non-Competitive) inhibitory pattern at 0.001 g/ml and exhibit uncompetitive inhibitory pattern at the 0.1 g/ml. The results of antioxidant potential showed EC50 for F.haussknechtii flower and H.acutifolium leaves equalled 2.37 and 0.96 mg/ml, respectively. Conclusions:The hexane extract of the F.haussknechtii flower, and H.acutifolium flower and leaf organ have a significant inhibitory effect on the activity of α-Glucosidase, DPPH free radical scavenging activities and reducing power. So they are good resources for extraction of medicinal compounds to control blood level of glucose after meal, in diabetic patients.
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