红三叶草原生质体的分离与培养及整株再生

J.W. Grosser, G.B. Collins
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引用次数: 38

摘要

已经开发了成功分离和培养红三叶草叶组织原生质体和细胞悬浮培养的方案。两种原生质源均获得了较高的电镀效率。通过体细胞胚发生实现了原生质体的整株再生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation and culture of Trifolium rubens protoplasts with whole plant regeneration

Protocols for the successful isolation and culture of Trifolium rubens protoplasts derived from leaf tissue and cell suspension cultures have been developed. High plating efficiencies were obtained with both sources of protoplasts. Whole plant regeneration from cultured protoplasts was achieved via somatic embryogenesis.

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