{"title":"红三叶草原生质体的分离与培养及整株再生","authors":"J.W. Grosser, G.B. Collins","doi":"10.1016/0304-4211(84)90220-7","DOIUrl":null,"url":null,"abstract":"<div><p>Protocols for the successful isolation and culture of <em>Trifolium rubens</em> protoplasts derived from leaf tissue and cell suspension cultures have been developed. High plating efficiencies were obtained with both sources of protoplasts. Whole plant regeneration from cultured protoplasts was achieved via somatic embryogenesis.</p></div>","PeriodicalId":20221,"journal":{"name":"Plant Science Letters","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0304-4211(84)90220-7","citationCount":"38","resultStr":"{\"title\":\"Isolation and culture of Trifolium rubens protoplasts with whole plant regeneration\",\"authors\":\"J.W. Grosser, G.B. Collins\",\"doi\":\"10.1016/0304-4211(84)90220-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Protocols for the successful isolation and culture of <em>Trifolium rubens</em> protoplasts derived from leaf tissue and cell suspension cultures have been developed. High plating efficiencies were obtained with both sources of protoplasts. Whole plant regeneration from cultured protoplasts was achieved via somatic embryogenesis.</p></div>\",\"PeriodicalId\":20221,\"journal\":{\"name\":\"Plant Science Letters\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1984-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0304-4211(84)90220-7\",\"citationCount\":\"38\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Science Letters\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0304421184902207\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Science Letters","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0304421184902207","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Isolation and culture of Trifolium rubens protoplasts with whole plant regeneration
Protocols for the successful isolation and culture of Trifolium rubens protoplasts derived from leaf tissue and cell suspension cultures have been developed. High plating efficiencies were obtained with both sources of protoplasts. Whole plant regeneration from cultured protoplasts was achieved via somatic embryogenesis.
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