激光自动荧光和染料增强光谱检测正常和癌变人肺细胞系和等离子体光热加热

M. E. Khosroshahi, M. Asemani, Payam Khoshkenar
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引用次数: 0

摘要

本工作描述了正常MRC-5和癌QU-DB人肺细胞系的激光自身荧光、染料增强荧光光谱的结果以及金纳米壳加热对细胞的影响。结果表明,MRC-5与QU-DB 80的强度比(ΔR)和形状因子(RP)分别在500 nm和590 nm处的初级峰和次级峰可以作为正常细胞和癌细胞的合适指标。在癌细胞中观察到约5nm的红移。最高气温约51度和39.5度?(S3, S4)和(S5, S6)分别达到C。损伤细胞的百分比和纳米壳的密度随着离光束中心径向距离的增加而减小。结果还表明,随着激光功率的增加,细胞凋亡程度增加,这是激光热疗质量和程度的关键因素。基体温度的变化被认为是由于较大比例的纳米颗粒的可能的热重叠、团聚和聚类。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Laser Auto Fluorescence and Dye-enhanced Spectroscopic Detection of Normal and Cancerous Human Lung Cell Lines and Plasmonic Photothermal Heating
This work describes the results of laser autofluorescence, dye-enhanced fluorescence spectroscopy of normal MRC-5 and cancerous QU-DB human lung cell lines and the effect of gold nanoshells heating on the cells. It was shown that the intensity ratio of MRC-5 to QU-DB 80 (ΔR) and the shape factor (RP) can be appropriate indicators of normal and cancerous cells for primary and secondary peaks at 500 nm and 590 nm respectively. About 5 nm red shift was observed in the case of cancerous cells. Highest temperatures of about 51 and 39.5?C were achieved for (S3, S4) and (S5, S6) respectively. The percentage of damaged cells and nanoshells density are expected to decrease with radial distance from the center of the beam. The results also indicated an increasing degree of apoptosis with increasing the laser power, which critically governs the quality and the degree of laser-hyperthermia. The matrix temperature is thought to vary due to possible thermal overlapping of a larger fraction of NSs, agglomeration and clustering.
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