{"title":"芽孢Septoria Nodorum在培养和发病过程中产生的多糖降解酶","authors":"Paolo Magro","doi":"10.1016/0304-4211(84)90204-9","DOIUrl":null,"url":null,"abstract":"<div><p><em>Septoria nodorum</em> produced cell wall-degrading enzymes (CWDE) (oolygalacturonase (PG), xylanase (XY) and cellulase (CX)) both in mineral medium supplemented with wheat cell walls and in inoculated wheat leaves. The same in vitro and in vivo isoenzyme pattern for PG (pI 6.2 and 9.4) and XY (pI 5.2) was exhibited by isoelectric focusing resolution. CX was present in culture filtrates with one poorly resolved peak (pI 4.3–4.7). In infected tissues, an additional peak at pI 7.8 appeared. Some characteristics of <em>S. nodorum</em> polysaccharidases were compared with those of other leaf spot pathogens.</p></div>","PeriodicalId":20221,"journal":{"name":"Plant Science Letters","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0304-4211(84)90204-9","citationCount":"17","resultStr":"{\"title\":\"Production of polysaccharide-degrading enzymes by Septoria Nodorum in culture and during pathogenesis\",\"authors\":\"Paolo Magro\",\"doi\":\"10.1016/0304-4211(84)90204-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><em>Septoria nodorum</em> produced cell wall-degrading enzymes (CWDE) (oolygalacturonase (PG), xylanase (XY) and cellulase (CX)) both in mineral medium supplemented with wheat cell walls and in inoculated wheat leaves. The same in vitro and in vivo isoenzyme pattern for PG (pI 6.2 and 9.4) and XY (pI 5.2) was exhibited by isoelectric focusing resolution. CX was present in culture filtrates with one poorly resolved peak (pI 4.3–4.7). In infected tissues, an additional peak at pI 7.8 appeared. Some characteristics of <em>S. nodorum</em> polysaccharidases were compared with those of other leaf spot pathogens.</p></div>\",\"PeriodicalId\":20221,\"journal\":{\"name\":\"Plant Science Letters\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1984-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0304-4211(84)90204-9\",\"citationCount\":\"17\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Science Letters\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0304421184902049\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Science Letters","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0304421184902049","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Production of polysaccharide-degrading enzymes by Septoria Nodorum in culture and during pathogenesis
Septoria nodorum produced cell wall-degrading enzymes (CWDE) (oolygalacturonase (PG), xylanase (XY) and cellulase (CX)) both in mineral medium supplemented with wheat cell walls and in inoculated wheat leaves. The same in vitro and in vivo isoenzyme pattern for PG (pI 6.2 and 9.4) and XY (pI 5.2) was exhibited by isoelectric focusing resolution. CX was present in culture filtrates with one poorly resolved peak (pI 4.3–4.7). In infected tissues, an additional peak at pI 7.8 appeared. Some characteristics of S. nodorum polysaccharidases were compared with those of other leaf spot pathogens.
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