R. Dwiyani, Hestin Yuswanti, N. N. A. Mayadewi, Yuyun Fitriani
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引用次数: 1
摘要
对几种芽诱导培养基上生长的草莓(Fragaria x ananassa Duch)的茎尖培养进行了研究。本研究的目的是在草莓微繁建立阶段，寻找最适合草莓茎尖培养产芽的培养基。试验采用完全随机设计，4个诱导芽的培养基型处理，10个重复，每个重复1瓶，6-8个外植体。处理总结如下:T1 = MS;T2 = MS + 2ppm BAP + 0.01 ppm NAA;T3 = MS + 1ppm TDZ;T4 = WPM + 2ppm BAP + 0.01 ppm NAA。观察的参数为芽出天数、每外植体平均芽数和每外植体平均叶数。综上所述，在本研究使用的培养基中，添加1ppm噻脲(TDZ)的MS培养基是最适合草莓穗尖外植体产芽的培养基。结果表明，该处理的出芽时间最早，芽叶数量最多。
RUNNER-TIP CULTURE OF STRAWBERRY (Fragaria x ananassa Duch) GROWN ON SEVERAL SHOOT-INDUCTION MEDIUM
A research regarding “Runner-tip culture of strawberry (Fragaria x ananassa Duch) Grown on Several Shoot-induction Medium” has been investigated. The objective of the research was to find out the most suitable medium for shoot production from runner-tip culture of strawberry at establishment step of micropropagation. The research was laid out in a Completely Randomized Design, 4 treatments of medium type for shoot induction and 10 replication, each was represented by one (1) bottle with 6-8 explants. The treatments were summarized as follows: T1 = MS ; T2 = MS + 2 ppm BAP + 0.01 ppm NAA; T3 = MS + 1 ppm of TDZ; T4 = WPM + 2 ppm BAP + 0.01 ppm NAA. The parameters observed were days of the bud emergence, the average number of shoots per explant, and the average number of leaves per explant. It can be concluded that among medium used in the current research, the medium of MS added with 1 ppm thidiazuron (TDZ) was the most suitable medium for shoot production of strawberry from the explant of runner-tips. The treatment was resulted in the earliest time of bud emergence, and producing the highest number of shoots and leaves.
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