一种诊断肺囊虫肺炎的实时聚合酶链反应方法的开发和验证

The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien des Maladies Infectieuses et de la Microbiologie Médicale Pub Date : 2015-01-01 DOI:10.1155/2015/138787

D. Church, A. Ambasta, A. Wilmer, Holly Williscroft, G. Ritchie, D. Pillai, S. Champagne, D. Gregson

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引用次数: 14

摘要

肺囊虫性肺炎是由肺囊虫引起的，这是一种机会性真菌病原体。目前，许多临床微生物实验室依赖于直接显微镜检测耶氏弧菌。作者讨论了一种快速检测肺囊虫肺炎的定性杰氏疟原虫实时聚合酶链反应方法的验证、临床和实验室发展。此外，还将新技术与现有的金标准免疫荧光法进行了比较。

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Development and validation of a Pneumocystis jirovecii real-time polymerase chain reaction assay for diagnosis of Pneumocystis pneumonia

Pneumocystis pneumonia is caused by Pneumocystis jirovecii, an opportunistic fungal pathogen. Presently, many clinical microbiology laboratories rely on direct microscopic detection of P jirovecii. The validation, and clinical and laboratory development of a qualitative P jirovecii real-time polymerase chain reaction assay for the rapid detection of Pneumocystis pneumonia is discussed by the authors. In addition, this new technique is compared with the existing gold-standard immunofluorescence assay.

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The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien des Maladies Infectieuses et de la Microbiologie Médicale

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