High-performance liquid chromatography analysis of gallic acid and kaempferol in chloroform and ethanol extract of Cassia hirsuta seeds

Aim: The present study aim was to analyze the gallic acid and kaempferol in chloroform and ethanol extract of Cassia hirsuta seeds by high-performance liquid chromatography (HPLC) method. Materials and Methods: In this study, the HPLC analysis of gallic acid and kaempferol was done by selected HPLC methods and these were validated for linearity, precision, limit of detection, limit of quantitation, repeatability, and reproducibility. The corresponding concentration of gallic acid and kaempferol against respective peak area values was determined using the gallic acid and kaempferol calibration curves, respectively. Results: In results, it was found that the linearity was good with regression equation y=36934x−10162 and y=61050x−9395.5 for gallic acid and kaempferol, respectively, and the correlation coefficient (R2) was 0.9982 and 0.9992, respectively. Percentage relative standard deviation of 1.6% and 0.71% for gallic acid and kaempferol, respectively, indicates the high precision. Ethanol extract of C. hirsuta shows high content of both gallic acid and kaempferol as compared to chloroform extract of C. hirsuta. Conclusion: In the present study, gallic acid and kaempferol content were determined in ethanol and chloroform extract of C. hirsuta. The developed HPLC methods have enabled rapid, linear, accurate, and reproducible analysis in these two extracts of C. hirsuta.