决明子氯仿和乙醇提取物中没食子酸和山奈酚的高效液相色谱分析

M. Shalavadi
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引用次数: 0

摘要

目的:采用高效液相色谱法分析决明子氯仿及乙醇提取物中没食子酸和山奈酚的含量。材料与方法:本研究选择HPLC方法对没食子酸和山奈酚进行HPLC分析,并对其线性度、精密度、检出限、定量限、重复性和再现性进行验证。利用没食子酸和山奈酚的标定曲线分别测定了没食子酸和山奈酚的峰面积对应浓度。结果:没食子酸和山奈酚的线性关系良好,分别为y=36934x−10162和y=61050x−9395.5,相关系数(R2)分别为0.9982和0.9992。没食子酸和山奈酚的相对标准偏差分别为1.6%和0.71%,具有较高的精密度。毛缕草乙醇提取物的没食子酸和山奈酚含量均高于氯仿提取物。结论:本研究测定了毛草乙醇和氯仿提取物中没食子酸和山奈酚的含量。所建立的高效液相色谱分析方法能够快速、线性、准确、重现性好。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
High-performance liquid chromatography analysis of gallic acid and kaempferol in chloroform and ethanol extract of Cassia hirsuta seeds
Aim: The present study aim was to analyze the gallic acid and kaempferol in chloroform and ethanol extract of Cassia hirsuta seeds by high-performance liquid chromatography (HPLC) method. Materials and Methods: In this study, the HPLC analysis of gallic acid and kaempferol was done by selected HPLC methods and these were validated for linearity, precision, limit of detection, limit of quantitation, repeatability, and reproducibility. The corresponding concentration of gallic acid and kaempferol against respective peak area values was determined using the gallic acid and kaempferol calibration curves, respectively. Results: In results, it was found that the linearity was good with regression equation y=36934x−10162 and y=61050x−9395.5 for gallic acid and kaempferol, respectively, and the correlation coefficient (R2) was 0.9982 and 0.9992, respectively. Percentage relative standard deviation of 1.6% and 0.71% for gallic acid and kaempferol, respectively, indicates the high precision. Ethanol extract of C. hirsuta shows high content of both gallic acid and kaempferol as compared to chloroform extract of C. hirsuta. Conclusion: In the present study, gallic acid and kaempferol content were determined in ethanol and chloroform extract of C. hirsuta. The developed HPLC methods have enabled rapid, linear, accurate, and reproducible analysis in these two extracts of C. hirsuta.
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