鱼类孵化场微藻种群长期储存替代技术的发展

Ajan Chellappan, Praba Thangamani, Shyni Markose, Selvaraj Thangaswamy, Uma Ganapathi, Citarasu Thavasimuthu, Michaelbabu Mariavincent
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引用次数: 1

摘要

藻类培养的维护面临着与气候变化、污染、设备故障、电力故障、不明原因的崩溃、实验室设施不完善有关的几个问题。传统的微藻保存是通过连续传代培养的方法,这是费力的,昂贵的,并且有很高的培养污染风险。虽然一般的传代培养可能无法持久地保持独特的特征,但冷冻保存方法可以更好地防止所需特征的改变。由于每隔一段时间更换液氮的成本效益和保存所需的技术人员。目的是寻找一种低成本的微藻保存技术。采用常见的冷冻保护剂(甲醇、二甲二甲砜、乙二醇和甘油)在-196°C和-20°C条件下保存了6个月的微藻Nannochloropsis salina、Chlorella volutis、Cheatoceros gracilis、Dunaliella sp.和Amphora sp.。解冻后评估微藻的生存能力,并测量细胞计数。在- 20°C和- 196°C条件下,盐绿纳米藻、体积小球藻、杜氏藻和双耳藻在6个月的孵育期中表现出良好的反应,存活能力变化不大,而细毛小毛藻仅在- 196°C条件下再生,在- 20°C条件下没有恢复。在本研究中,一种新的液氮保存方法已经被标准化,这种新方法可以为小型鱼类孵化场和微藻养殖场带来福利。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Development of Alternative Technology for the Long-term Storage of Microalgal Stock Culture in Fish Hatcheries
Maintenance of algal culture faces several problems associated with climate change, contaminations, equipment failures, power failures, unexplained crashes, poor lab facilities. Microalgae are traditionally preserved by serial subculture methods that are laborious, costly, and high risk of culture contamination. While unique characteristics may not be durably maintained with general subculture, cryopreservation methods better prevent alterations from desired characteristics. Because of the cost-effective in replacing of liquid nitrogen at every interval of time and technical person needed for the preservation. The objective is to find out an alternative and a low-cost technology for the preservation of microalgae Stock culture. The microalgae Nannochloropsis salina, Chlorella volutis, Cheatoceros gracilis, Dunaliella sp. and Amphora sp., were preserved using common cryoprotectants (methanol, DMSO, ethylene glycol and glycerol) for 6 months at –196°C and –20°C. The viabilities of the microalgae were assessed after thawing, and cell counts were measured. The preserved algae Nannochloropsis salina, Chlorella volutis, Dunaliella sp. and Amphora sp. evoked good responses with negligible changes in their survivability in 6 months incubation period when preserved at –20°C and – 196°C, while the Cheatoceros gracilis regenerate only in –196°C but not restored in –20°C. In this study, an alternative method for liquid nitrogen preservation has been standardized and this new method can be a boon for small level fish hatcheries and microalgal stock holders.
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