迷迭香酸对非酒精性脂肪性肝炎小鼠肝细胞炎症变化的保护作用:环氧化酶2/前列腺素E2通路

Tahereh Komeili-Movahhed, A. Moslehi
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引用次数: 0

摘要

背景和目的:非酒精性脂肪性肝炎(non - alcoholic Steatohepatitis, NASH)是一种常见的肝脏疾病,其特征是肝脏脂肪堆积过多(脂肪变性)和炎症。炎症是这种疾病的主要原因之一,它通过不同的途径发生。迷迭香酸(RA)是一种抗氧化和抗炎产品,对改善肝损伤有积极作用。本研究旨在通过影响环氧化酶-2/前列腺素E2 (COX-2/PEG2)通路和基质金属蛋白酶-9 (MMP-9)通路,评价RA对雄性小鼠NASH肝炎症的保护作用。方法:采用C57/BL6雄性小鼠24只。他们被分成四组,每组6人,包括;对照组、RA、NASH和NASH+RA。NASH由蛋氨酸/胆碱缺乏饮食诱导8周。RA每天腹腔注射,连续8周。8周后处死,取肝。苏木精和伊红染色进行组织学评价,实时荧光定量PCR和酶联免疫吸附试验进行分子研究。结果:RA可显著降低肝脏脂肪变性、炎症及MMP-9、COX-2的表达,导致PEG2水平显著降低。结论:RA可能通过降低COX-2酶和MMP-9的表达以及PEG2的浓度来减轻雄性小鼠肝脏炎症,从而减轻NASH。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Protective Effect of Rosmarinic Acid on Inflammatory Changes of Hepatic Cells in Non-alcoholic Steatohepatitis in Mice: Cyclooxygenase 2/ Prostaglandin E2 Pathway
Background and Objectives: Nonalcoholic Steatohepatitis (NASH) is one of the common liver diseases, characterized by excessive lipid accumulation (steatosis) and inflammation in the liver. Inflammation is one of main reasons for this disease which takes place from different pathways. Rosmarinic Acid (RA) is an antioxidant and anti-inflammatory product with positive effects on the improvement of liver injuries. This study aims to evaluate protective effect of RA against liver inflammation caused by NASH in male mice through affecting cyclooxygenase-2/ Prostaglandin E2 (COX-2/PEG2) pathway and Matrix Metalloproteinase-9 (MMP-9). Methods: In this study, 24 C57/BL6 male mice were used. They were divided into four groups of 6 including; Control, RA, NASH, and NASH+RA. The NASH was induced by a methionine/choline-deficient diet for 8 weeks. The RA was administrated intraperitoneally and daily for 8 weeks. After 8 weeks, the animals were sacrificed and their liver was removed. hematoxylin and eosin staining was preformed for histological evaluation and real-time PCR and ELISA assays were used for molecular studies. Results: The RA significantly reduced hepatic steatosis and inflammation as well as the expression of MMP-9 and COX-2 which led to the significant reduction of PEG2 level. Conclusion: It seems that RA can reduce liver inflammation and thereby attenuate NASH in male mice by reducing the expression of COX-2 enzyme and MMP-9 and the concentration of PEG2.
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