利用线粒体细胞色素c氧化酶亚基I DNA条形码序列首次对土耳其兼性丝虫病剂Eristalis tenax进行分子表征

IF 0.5 4区农林科学 Q4 VETERINARY SCIENCES

Turkish Journal of Veterinary & Animal Sciences Pub Date : 2020-03-01 DOI:10.3906/vet-1911-82

Mübeccel Okur, A. Yıldırım, A. Inci

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引用次数: 0

摘要

本报告是首次对从土耳其安纳托利亚中部开塞利省牛舍中采集的兼性蝇蛆病剂Eristalis tenax进行分子鉴定。本研究共收集成蝇标本10份。在体视显微镜下通过形态学特征鉴定所有蝇类均为雌性成虫。用PCR方法从田鼠标本的基因组DNA提取物中扩增出线粒体细胞色素氧化酶1 CO1基因的条形码区，并进行分子鉴定和系统发育分析。所有分离株的CO1序列均不存在多态性，均为单倍型。在由检测到的单倍型序列和GenBank中来自不同国家的E. tenax序列组成的CO1数据集中，单倍型和核苷酸多样性分别为0.775和0.002。新发现的单倍型与来自澳大利亚和加拿大的单倍型聚集在一个单系分支中，与澳大利亚的E. tenax分离物JN991985的同源性为100.0%。种间遗传差异在5.7% ~ 7.9%之间。

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First molecular characterization of the facultative myiasis agent Eristalis tenax in Turkey using mitochondrial cytochrome c oxidase subunit I DNA barcoding sequences

This report is the first molecular characterization of the facultative myiasis agent Eristalis tenax collected from cattle barns in Kayseri province of Central Anatolia, Turkey. A total of 10 adult fly specimens were included in the study. All flies were identified as female adults of E. tenax by morphologic characteristics under a stereomicroscope. The barcode region of the mitochondrial cytochrome oxidase 1 CO1 gene was amplified with PCR from the genomic DNA extracts of E. tenax specimens for molecular identification and phylogenetic analyses. There was no polymorphism among the CO1 sequences of all isolates leading to the detection of a single haplotype for E. tenax. Haplotype and nucleotide diversities in the CO1 data set comprised of the sequence of the detected haplotype and the published sequences of E. tenax from different countries in GenBank were determined as 0.775 and 0.002, respectively. The newly characterized haplotype in this study clustered with the haplotypes from Australia and Canada in a monophyletic clade and exhibited a 100.0% identity to the Australian E. tenax isolate JN991985 . Interspecific genetic differences between E. tenax and other Eristalis species were in the range of 5.7% to 7.9%.

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来源期刊

Turkish Journal of Veterinary & Animal Sciences 农林科学-兽医学

CiteScore

1.30

自引率

0.00%

发文量

审稿时长

24 months

期刊介绍： The Turkish Journal of Veterinary and Animal Sciences is published electronically 6 times a year by the Scientific and Technological Research Council of Turkey (TÜBİTAK). Accepts English-language manuscripts on all aspects of veterinary medicine and animal sciences. Contribution is open to researchers of all nationalities. Original research articles, review articles, short communications, case reports, and letters to the editor are welcome. Manuscripts related to economically important large and small farm animals, poultry, equine species, aquatic species, and bees, as well as companion animals such as dogs, cats, and cage birds, are particularly welcome. Contributions related to laboratory animals are only accepted for publication with the understanding that the subject is crucial for veterinary medicine and animal science. Manuscripts written on the subjects of basic sciences and clinical sciences related to veterinary medicine, nutrition, and nutritional diseases, as well as the breeding and husbandry of the above-mentioned animals and the hygiene and technology of food of animal origin, have priority for publication in the journal. A manuscript suggesting that animals have been subjected to adverse, stressful, or harsh conditions or treatment will not be processed for publication unless it has been approved by an institutional animal care committee or the equivalent thereof. The editor and the peer reviewers reserve the right to reject papers on ethical grounds when, in their opinion, the severity of experimental procedures to which animals are subjected is not justified by the scientific value or originality of the information being sought by the author(s).