利用平板自动合成器获得向导RNA的工艺优化

Q4 Chemistry

Proceedings of the National Academy of Sciences of Belarus, Chemical Series Pub Date : 2022-11-29 DOI:10.29235/1561-8331-2022-58-4-398-406

E. Ulashchik, T. P. Akhlamionok, P. Y. Baryshchyk, O. Sharko, V. Shmanai

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引用次数: 0

摘要

采用ASM-2000型自动合成仪，在500 nmol的条件下制备了CRISPR/Cas系统所需的向导RNA寡核苷酸。研究了合成RNA寡核苷酸的分离、纯化和分析控制方法。改进后的技术已用于制备CRISPR Cas12a系统的引导rna。

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Optimization of the technology for obtaining guide RNA using plate automatic synthesizer

A synthetic protocol was developed and optimal reagents have been selected for obtaining guide RNA oligonucleotides for the CRISPR/Cas system using ASM-2000 automatic synthesizer in 500 nmol scale. Methods for the isolation, purification and analytical control of synthetic RNA oligonucleotides have been developed. The improved technology has been used for preparation of guide RNAs for the CRISPR Cas12a system.

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来源期刊

Proceedings of the National Academy of Sciences of Belarus, Chemical Series Chemistry-Chemistry (miscellaneous)

CiteScore

0.30

自引率

0.00%

发文量