子宫内膜异位症相关卵巢交界性和恶性上皮肿瘤中的KRAS突变

IF 0.4 Q4 ONCOLOGY
S. Sarmadi, N. Izadi-mood, Marzieh Fakhari, R. Shahsiah, R. Miri, Maniya Mozafari
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引用次数: 0

摘要

背景:子宫内膜异位症是一种可转化为卵巢肿瘤的女性常见病。KRAS突变是许多恶性肿瘤发生的关键。目的:在本研究中,我们研究了KRAS突变在子宫内膜异位症相关卵巢交界性和恶性上皮肿瘤中的作用。方法:对连续42例最终诊断为子宫内膜异位症相关的交界性和恶性上皮性卵巢肿瘤患者的标本进行组织病理学回顾,其中12例交界性上皮性肿瘤和30例卵巢上皮性癌。对所有病例进行肿瘤类型、分化和同时存在子宫内膜异位症或非典型子宫内膜异位症的评估。从所选石蜡块中提取DNA,评估密码子12和13的突变。结果:42例患者中有6例由于PCR研究的基因组DNA质量不合格,其余36例患者中有6例发生KRAS突变,其中2例发生在第12密码子第2碱基(G→T)突变,3例发生在第12密码子第2碱基G→A突变,1例发生在第12密码子第1碱基G→T突变。结论:我们评估了与子宫内膜异位症相关的卵巢上皮肿瘤谱中KRAS突变的治疗方法,包括靶向治疗。我们的研究结果提示KRAS突变与子宫内膜异位症相关的卵巢交界性和恶性肿瘤之间可能存在联系,但没有令人信服的证据证明这种联系是明确的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
KRAS Mutation in Endometriosis-Associated Ovarian Borderline and Malignant Epithelial Tumors
Background: Endometriosis is a common disease among women with the capacity to transform into ovarian neoplasms. KRAS mutation is a keystone in tumor-genesis of many malignant neoplasms. Objectives: In the current study, we investigated KRAS mutations in endometriosis-associated ovarian borderline and malignant epithelial tumors. Methods: The specimens of 42 consecutive patients undergoing a surgical procedure whose final diagnosis comprised endometriosis-associated borderline and malignant epithelial ovarian tumors including 12 borderline epithelial tumors and 30 ovarian epithelial carcinomas were histopathologically reviewed. All cases were evaluated regarding the type of tumor, differentiation and simultaneous presence of endometriosis or atypical endometriosis. DNA extraction from the selected paraffin block was done and mutation of codons 12 and 13 was assessed. Results: Due to the quality of genomic DNA for PCR study was not acceptable in 6 out of 42 cases, among remaining 36 cases, KRAS mutation was observed in 6 cases including 2 cases with mutations in 2nd base of 12th codon (G→T), 3 cases with substitution of G→A in the 2nd base of 12th codon, and one with substitution of G→T in the 1st base of 12th codon. Conclusions: We evaluated the KRAS mutation in the spectrum of ovarian epithelial tumors associated with endometriosis for treatment approaches including targeted therapies. Our results suggested a possible link between KRAS mutation and endometriosis-associated ovarian borderline and malignant tumors but there was no convincing evidence to prove a definite linkage.
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来源期刊
CiteScore
1.10
自引率
0.00%
发文量
67
期刊介绍: International Journal of Cancer Management (IJCM) publishes peer-reviewed original studies and reviews on cancer etiology, epidemiology and risk factors, novel approach to cancer management including prevention, diagnosis, surgery, radiotherapy, medical oncology, and issues regarding cancer survivorship and palliative care. The scope spans the spectrum of cancer research from the laboratory to the clinic, with special emphasis on translational cancer research that bridge the laboratory and clinic. We also consider original case reports that expand clinical cancer knowledge and convey important best practice messages.
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