克氏原螯虾酚氧化酶原和MAPK/Nrf2通路对镉胁迫的响应

IF 0.9 4区 生物学 Q3 MARINE & FRESHWATER BIOLOGY
K. Wei, Junxian Yang, Chang-Khil Song
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引用次数: 5

摘要

在甲壳类动物中,免疫应答是一个由多种分子、细胞和信号通路组成的复杂网络。镉诱导的免疫抑制已被广泛研究,但其对小龙虾proPO的作用机制尚不清楚。本研究通过对克氏原螯虾(Procambarus clarkii)进行亚致死Cd2+浓度(分别为1、5和10 mg/L)处理96 h,研究了血淋巴中proPO活性与肝胰脏p38MAPK和Nrf2活性的关系。结果表明,血淋巴THC和proPO水平显著降低,肝胰脏ROS含量显著升高(P < 0.01),且呈剂量依赖性和时间依赖性。免疫组化检测发现,Cd2+处理组p38MAPK和Nrf2的表达和激活均较对照组显著升高,且P -p38/p38和Nrf2水平与proPO活性密切相关(P < 0.01)。肝胰腺可能通过ros介导的MAPK/Nrf2途径参与系统性proPO活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The responses of prophenoloxidase and MAPK/Nrf2 pathway to cadmium stress in red swamp crayfish Procambarus clarkii
ABSTRACT The immune response is a complex network composed of various molecules, cells and signaling pathways in crustacean. Cadmium-induced immunosuppression has been widely researched, but its underlying mechanism on crayfish proPO remains to be elucidated. In this study, the relationship between proPO activity in haemolymph and the activation of p38MAPK and Nrf2 in hepatopancreas was evaluated after red swamp crayfish Procambarus clarkii were subjected to sub-lethal Cd2+ concentrations (i.e. 1,5 and 10 mg/L) for 96 h. Results indicated that there was a significant decrease in haemolymph THC and proPO levels accompanied by the increasing contents of hepatopancreas ROS (P < 0.01) in a dose- and time-dependent manner. By IHC detection, the expression and activation of p38MAPK and Nrf2 were remarkably increased in Cd2+ treatment groups compared with the control group, and the level of both p-p38/p38 and Nrf2 was closely related to proPO activity (P < 0.01). Hepatopancreas might be implicated in systemic proPO activity via ROS-mediated MAPK/Nrf2 pathway.
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来源期刊
Marine and Freshwater Behaviour and Physiology
Marine and Freshwater Behaviour and Physiology 生物-海洋与淡水生物学
CiteScore
2.10
自引率
0.00%
发文量
9
审稿时长
>12 weeks
期刊介绍: Marine and Freshwater Behaviour and Physiology is devoted to the publication of papers covering field and laboratory research into all aspects of the behaviour and physiology of all marine and freshwater animals within the contexts of ecology, evolution and conservation. As the living resources of the world’s oceans, rivers and lakes are attracting increasing attention as food sources for humans and for their role in global ecology, the journal will also publish the results of research in the areas of fisheries biology and technology where the behaviour and physiology described have clear links to the contexts mentioned above. The journal will accept for publication Research Articles, Reviews, Rapid Communications and Technical Notes (see Instructions for authors for details). In addition, Editorials, Opinions and Book Reviews (invited and suggested) will also occasionally be published. Suggestions to the Editor-In-Chief for Special Issues are encouraged and will be considered on an ad hoc basis. With the goal of supporting early career researchers, the journal particularly invites submissions from graduate students and post-doctoral researchers. In addition to recognising the time constraints and logistical limitations their research often faces, and their particular need for a prompt review process, accepted articles by such researchers will be given prominence within the journal (see Instructions for authors for details).
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