表面活性剂吐温80对醋酸罗沙替丁和载醋酸罗沙替丁壳聚糖纳米颗粒与溶菌酶结合的影响

Mohsen T.A. Qashqoosh, Faiza A.M. Alahdal, Yahiya Kadaf Manea, S. Zubair, S. Naqvi
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引用次数: 0

摘要

药物与蛋白质的结合是一个很有吸引力的研究课题。为了研究RxAc- csnp在生理条件下的释放及其与溶菌酶的结合,在Tween 80 (Tw80)表面活性剂存在下,制备了壳聚糖(Cs)和醋酸罗沙替丁(RxAc)纳米复合材料。在csnp中加入Tw80增加了RxAc的体外释放。Stern-Volmer图和热力学结果表明,Lyz与RxAc和Lyz与RxAc- csnps的结合机理为静态机制,两种体系的主要作用力均为氢键和范德华力,表明两种体系的结合反应是自发的、放热的、焓驱动的。同步荧光和CD结果表明,RxAc和RxAc- csnp引起了Lyz二级结构的变化。还发现Tw80的加入影响了药物与蛋白质的结合常数。最后,分子对接的结果也与其他技术的结果一致。因此,制备的负载RxAc的壳聚糖纳米颗粒可作为抗溃疡药物设计和应用的有效策略。总之,本研究可以为未来抗溃疡药物的设计提供重要的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Influence of Tween 80 Surfactant on the Binding of Roxatidine Acetate and Roxatidine Acetate–loaded Chitosan Nanoparticles to Lysozyme
The drug binding to protein is an attractive research topic. In order to assess the release of RxAc-CsNPs and their binding with lysozyme under physiological conditions, nanocomposite materials based on chitosan (Cs) and Roxatidine acetate (RxAc) in the presence Tween 80 (Tw80) surfactant were developed. The addition of Tw80 to CsNPs increased RxAc release in vitro. In this work, Stern–Volmer plot and thermodynamic results indicated that the mechanism of Lyz with RxAc and Lyz with RxAc-CsNPs was static mechanism and the main forces in both systems were hydrogen bonding and Van der Waals forces, which indicated that the binding reaction in both systems is spontaneous, exothermic and enthalpically driven. Synchronous fluorescence and CD results indicated that the RxAc and RxAc-CsNPs cause change in the secondary construction of Lyz. It was also found that the addition of Tw80 affects the binding constant of drug with protein. Finally, the molecular docking results have also been in accordance with the results of other techniques. Hence, the developed RxAc loaded Chitosan nanoparticles could be used as an effective strategy for designing and application of the antiulcer drugs. Altogether, the present study can provide an important insight for the future designing of antiulcer drugs.
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