针对免疫相关动脉粥样硬化基因的sirna设计:计算研究

Q3 Biochemistry, Genetics and Molecular Biology

Turkish Computational and Theoretical Chemistry Pub Date : 2023-07-04 DOI:10.33435/tcandtc.1246320

H. Al-Madhagi

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引用次数: 0

摘要

1.1目的动脉粥样硬化是一种慢性、免疫相关的疾病，在全球范围内具有很高的死亡率。目前研究的目的是利用生物信息学工具通过特定的siRNA靶向这些基因。1.2方法从NCBI数据库中检索C1QA和ITBG2基因序列的rnas，设计8个sirna。通过OligoCalc网络界面计算sirna的GC%和Tm。此外，利用DuplexFold和HDock检测sirna与相应靶序列的杂交能以及与argonaute 2蛋白的对接。1.3结果设计的sirna具有可接受的GC含量和Tm值。此外，杂交能和对接分数对上述基因的表达均有极显著的阻断作用。1.4结论所设计的sirna是沉默免疫介导的动脉粥样硬化基因的较好候选者，值得进一步研究。

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Design of siRNAs Against Immune-Implicated Atherosclerosis Genes: Computational Study

1.1 Objective Atherosclerosis is a chronic, immune-implicated, disease with high numbers of mortality globally. The aim of the current study is to target these genes by specific siRNA utilizing bioinformatics tools. 1.2 Methods 8 siRNAs were designed via RNAxs from C1QA and ITBG2 gene sequences retrieved from NCBI database. GC% and Tm of siRNAs were calculated through OligoCalc web interface. In addition, hybridization energy of siRNAs with the corresponding target sequences as well as docking to argonaute 2 protein were performed using DuplexFold and HDock. 1.3 Results The designed siRNAs exhibited acceptable GC content and Tm values. Besides, the hybridization energy and docking scores were highly significant to block the expression of the mentioned genes. 1.4 Conclusion The designed siRNAs are superior candidates for silencing immune-mediated atherosclerotic genes which deserve further consideration.

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来源期刊

Turkish Computational and Theoretical Chemistry Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (miscellaneous)

CiteScore

2.40

自引率

0.00%

发文量