β-环糊精与比马前列素包合作用的荧光光谱研究:对绿色分析应用的挑战

M. I. Walash, S. Toubar, Maha M AbouEl-Alamin, Maha A Elabd, N. N. Salama
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引用次数: 0

摘要

在β-环糊精存在和无β-环糊精存在的情况下,对马马前列素包合作用的荧光研究表明,在β-环糊精存在的情况下,马马前列素的天然荧光显著增加。比马托前列素与β-环糊精的主-客体相互作用荧光光谱显示形成包合物,其化学计量比为1:1。利用双马前列素在疏水β-环糊精腔内包合时的天然荧光变化来计算其缔合常数。用荧光法测定了在1% (w/v) β-环糊精存在和不存在的情况下比马前列素的含量。在所研究的药物在水中λex 217 nm激发后,在λem 285处显示出天然荧光。计算了β-CD存在和不存在情况下的量子产率[QY],发现它从0.26增加到0.31。对不同实验参数对药物荧光的影响进行了仔细的研究和优化。在β-CD不存在和不存在的情况下,样品在25 ~ 250 ng/mL和5 ~ 50 ng/mL范围内呈线性关系,检测限分别为0.05和0.006 ng/mL,定量限分别为0.18和0.02 ng/mL。根据ICH指南验证了所提出的方法,并有效地应用于所研究药物的眼科配方分析。所得结果与文献报道的方法进行了统计比较,显示出较高的准确度和精密度。所提出的方法对绿色具有挑战性。定性和定量指标揭示了优良的环保荧光法应用于QC实验室。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Spectrofluorimetric Study on Inclusion Interaction of β- Cyclodextrin with Bimatoprost: Challenging to Green Analytical Applications
Fluorescence study on inclusion interaction of bimatoprost in absence and presence of β-cyclodextrin shows significant increase in native fluorescence of bimatoprost in the presence of β-cyclodextrin. Fluorescence spectroscopy of host-guest interaction between bimatoprost and β-cyclodextrin shows formation of inclusion complex with 1:1 stoichiometric ratio. The changes of native fluorescence of bimatoprost on inclusion in the hydrophobic β-cyclodextrin cavity is used to calculate the association constant.The fluorimetric method was used for determination of bimatoprost in absence and presence of 1% (w/v) β-cyclodextrin. The studied drug shows native fluorescence at λem 285 after excitation at λex 217 nm in water. The quantum yield [QY] was calculated in absence and presence of β-CD and it was found to be increased from 0.26 to 0.31. The different experimental parameters affecting the fluorescence of the drug was carefully studied and optimized. Linearity was over the range of 25 – 250 ng/mL and 5 – 50 ng/mL in absence and presence of β-CD, respectively with detection limits of 0.05 and 0.006 ng/mL, and quantitation limits of 0.18, and 0.02 ng/mL in absence and presence of β-CD, respectively. The proposed methods were validated as per ICH guidelines, and were effectively applied to analysis of studied drug in its ophthalmic formulation. The results obtained were statistically compared with the reported method revealing high accuracy and good precision. The proposed methods are challenging to green. Qualitative and quantitative metrics revealed excellent eco-friendly fluorimetric method for application in QC laboratories.
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