辣木根水提物对Wistar大鼠酒精性血液中毒的抗瘙痒作用

G. Ekpo, E. Ofem, S. Bassey, K. Abam, I. Arikpo, J. Enobong, S. Ufot, V. Ekam
{"title":"辣木根水提物对Wistar大鼠酒精性血液中毒的抗瘙痒作用","authors":"G. Ekpo, E. Ofem, S. Bassey, K. Abam, I. Arikpo, J. Enobong, S. Ufot, V. Ekam","doi":"10.9734/IBRR/2019/V9I430104","DOIUrl":null,"url":null,"abstract":"Aim: The study is aimed at investigating the antinutritive effects of aqueous root extract of Moringa oleifera on haematological indices following sub-chronic consumption of alcohol in Wistar rats. \nMethods and Materials: Thirty (30) experimental rats weighing 100-120 g were randomly divided into 6 groups of 5 rats each. Group 1, the normal control group was given just rat feed and water, group 2, negative control was administered alcohol only (1.5 ml/kg body weight), rats in group 3, 4 and 5 received combined administration of alcohol 1.5 ml/kg body weight and 200, 400 and 600 mg/kg body weight of aqueous root extract of Moringa oleifera respectively, group 6 rats were administered 250 mg/body weight of extract only. At the end of fourteen days (14) the experimental rats were then sacrificed, and blood was collected for haematological analysis. \nResults: The result obtained shows a dose-dependent response in almost all the parameters, white blood cell (WBC) count increases significantly(P<0.05) with 600 mg/kg while dose 200 mg/kg and 400 mg/g shows no significant change. Red blood cells (RBC) decreases non-significantly (P<0.05) with increased amount of aqueous Moringa root extract.  Likewise, there was also a dose-dependent decrease in the haematological count and more noticeable with 200 mg/kg dose. It was also observed that mean corpuscular haemoglobin concentration (MCHC) and mean corpuscular volume (MCV) increases in all the treatment groups with significant observed in 400 mg/kg and 600 mg/kg. The increase in alcohol can bring about suppression of the blood cells production, enhance if there is reduction in the red blood cells, it, therefore, implies that the will be reduction in the oxygen-carrying capacity of the red blood cells, that would be carried to the tissues as well as the level of carbon dioxide returned to the lungs. Also, the PCV is involved in the transport of oxygen and absorption of the nutrient. Therefore, increase in PCV, shows a better transportation and thus results in an increase in both primary and secondary polycythemias. \nConclusion: This research work shows that the plant extract contains some bioactive component or phytochemical constituents that are capable of ameliorating the toxicity effect of alcohol on animal models.","PeriodicalId":13659,"journal":{"name":"International Blood Research & Reviews","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Antipuritive Effect of Aqueous Root Extracts of Moringa oliefera on Alcohol-induced Haematoxicity in Wistar Rats\",\"authors\":\"G. Ekpo, E. Ofem, S. Bassey, K. Abam, I. Arikpo, J. Enobong, S. Ufot, V. Ekam\",\"doi\":\"10.9734/IBRR/2019/V9I430104\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Aim: The study is aimed at investigating the antinutritive effects of aqueous root extract of Moringa oleifera on haematological indices following sub-chronic consumption of alcohol in Wistar rats. \\nMethods and Materials: Thirty (30) experimental rats weighing 100-120 g were randomly divided into 6 groups of 5 rats each. Group 1, the normal control group was given just rat feed and water, group 2, negative control was administered alcohol only (1.5 ml/kg body weight), rats in group 3, 4 and 5 received combined administration of alcohol 1.5 ml/kg body weight and 200, 400 and 600 mg/kg body weight of aqueous root extract of Moringa oleifera respectively, group 6 rats were administered 250 mg/body weight of extract only. At the end of fourteen days (14) the experimental rats were then sacrificed, and blood was collected for haematological analysis. \\nResults: The result obtained shows a dose-dependent response in almost all the parameters, white blood cell (WBC) count increases significantly(P<0.05) with 600 mg/kg while dose 200 mg/kg and 400 mg/g shows no significant change. Red blood cells (RBC) decreases non-significantly (P<0.05) with increased amount of aqueous Moringa root extract.  Likewise, there was also a dose-dependent decrease in the haematological count and more noticeable with 200 mg/kg dose. It was also observed that mean corpuscular haemoglobin concentration (MCHC) and mean corpuscular volume (MCV) increases in all the treatment groups with significant observed in 400 mg/kg and 600 mg/kg. The increase in alcohol can bring about suppression of the blood cells production, enhance if there is reduction in the red blood cells, it, therefore, implies that the will be reduction in the oxygen-carrying capacity of the red blood cells, that would be carried to the tissues as well as the level of carbon dioxide returned to the lungs. Also, the PCV is involved in the transport of oxygen and absorption of the nutrient. Therefore, increase in PCV, shows a better transportation and thus results in an increase in both primary and secondary polycythemias. \\nConclusion: This research work shows that the plant extract contains some bioactive component or phytochemical constituents that are capable of ameliorating the toxicity effect of alcohol on animal models.\",\"PeriodicalId\":13659,\"journal\":{\"name\":\"International Blood Research & Reviews\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-07-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Blood Research & Reviews\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.9734/IBRR/2019/V9I430104\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Blood Research & Reviews","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.9734/IBRR/2019/V9I430104","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1

摘要

目的:研究辣木根水提物对Wistar大鼠亚慢性饮酒后血液学指标的抗营养作用。方法与材料:选取体重100 ~ 120 g的实验大鼠30只,随机分为6组,每组5只。1组正常对照组只给予大鼠饲料和水,2组阴性对照组只给予酒精(1.5 ml/kg体重),3、4、5组大鼠分别给予酒精1.5 ml/kg体重和辣木根水提物200、400、600 mg/kg体重,6组大鼠只给予辣木根水提物250 mg/kg体重。14 d后处死实验大鼠,采集血液进行血液学分析。结果:所得结果几乎所有参数均呈剂量依赖性,600 mg/kg剂量组白细胞计数显著升高(P<0.05), 200 mg/kg和400 mg/g剂量组无显著变化。血红细胞(RBC)随辣木根水提物添加量的增加而显著降低(P<0.05)。同样,血液学计数也有剂量依赖性下降,200 mg/kg剂量时更为明显。各治疗组平均红细胞血红蛋白浓度(MCHC)和平均红细胞体积(MCV)均升高,其中400 mg/kg和600 mg/kg组显著升高。酒精的增加会抑制血细胞的生成,如果红细胞减少,则会增强,因此,这意味着红细胞携带氧气的能力会降低,这些氧气将被运送到组织中,同时二氧化碳的水平也会返回到肺部。此外,PCV还参与氧气的运输和营养物质的吸收。因此,PCV的增加表现出更好的运输,从而导致原发性和继发性红细胞增多症的增加。结论:该植物提取物含有一定的生物活性成分或植物化学成分,能够改善酒精对动物模型的毒性作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antipuritive Effect of Aqueous Root Extracts of Moringa oliefera on Alcohol-induced Haematoxicity in Wistar Rats
Aim: The study is aimed at investigating the antinutritive effects of aqueous root extract of Moringa oleifera on haematological indices following sub-chronic consumption of alcohol in Wistar rats. Methods and Materials: Thirty (30) experimental rats weighing 100-120 g were randomly divided into 6 groups of 5 rats each. Group 1, the normal control group was given just rat feed and water, group 2, negative control was administered alcohol only (1.5 ml/kg body weight), rats in group 3, 4 and 5 received combined administration of alcohol 1.5 ml/kg body weight and 200, 400 and 600 mg/kg body weight of aqueous root extract of Moringa oleifera respectively, group 6 rats were administered 250 mg/body weight of extract only. At the end of fourteen days (14) the experimental rats were then sacrificed, and blood was collected for haematological analysis. Results: The result obtained shows a dose-dependent response in almost all the parameters, white blood cell (WBC) count increases significantly(P<0.05) with 600 mg/kg while dose 200 mg/kg and 400 mg/g shows no significant change. Red blood cells (RBC) decreases non-significantly (P<0.05) with increased amount of aqueous Moringa root extract.  Likewise, there was also a dose-dependent decrease in the haematological count and more noticeable with 200 mg/kg dose. It was also observed that mean corpuscular haemoglobin concentration (MCHC) and mean corpuscular volume (MCV) increases in all the treatment groups with significant observed in 400 mg/kg and 600 mg/kg. The increase in alcohol can bring about suppression of the blood cells production, enhance if there is reduction in the red blood cells, it, therefore, implies that the will be reduction in the oxygen-carrying capacity of the red blood cells, that would be carried to the tissues as well as the level of carbon dioxide returned to the lungs. Also, the PCV is involved in the transport of oxygen and absorption of the nutrient. Therefore, increase in PCV, shows a better transportation and thus results in an increase in both primary and secondary polycythemias. Conclusion: This research work shows that the plant extract contains some bioactive component or phytochemical constituents that are capable of ameliorating the toxicity effect of alcohol on animal models.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信