Screening and simultaneous determination of 80 veterinary drugs residues in food by HPLC-MS/MS

A fast method of samples screening and determination of 80 veterinary drugs residues in food safety assessment is proposed. The solid-phase extraction for the extract purification in the sample preparation is excluded, at the same time the matrix effect is mitigated by the isotope-labeled standards using. The isotope dilution method allowed to exclude matrix calibration curves construction and calculate the concentration using correction coefficients found from analytes standard aqueous solutions and a limited number of isotope-labeled standards. The drugs residues limits of detection (cmin = 0,1 – 10 ng/g) allowed their determination at the maximum permissible levels for food.