Determination of Genetic Distance among Genotypes of Cucumber, Cucumis sativus L., Using ISSR Marks

This research was conducted in biotechnology laboratories of grain test and validation office – Ministry of Agriculture, 2016. Nine cucumber genotypes, represented pure local and imported classes,were cultivated to evaluate genetic diversity and determine genetic affinity among them by using 13 starters kind Inter Simple Sequence Repeats (‏ISSR), 10 of them showed an activity to give apolymorphism among the studied genotypes which give 94 alleles, the ratio of this polymorphism was 68%. The starter UBC862 gave higher bands of 15, while the starter A35 gave lower bands of 6. The study showed that genetic similarity of the genetic affinity ranged between 0.506 – 0769 in which the higher genetic affinity (0.769) was for V5 and V2 varieties followed by (0.743) for V6 and V3 varieties, while the lower genetic affinity (0.506) was for V8 and V9 varieties.