G. S. Djarkasi, Lana E. Lalujan, Erny J. N. Nurali, M. Sumual
{"title":"菖蒲的抗氧化活性","authors":"G. S. Djarkasi, Lana E. Lalujan, Erny J. N. Nurali, M. Sumual","doi":"10.1063/1.5125555","DOIUrl":null,"url":null,"abstract":"Karimenga (Acorus calamus) is an underutilized plant that contains bioactive compounds such as phenolics, flavonoids, and terpenoids. The accumulation of phenolic compounds varies in each part of the plant; hence the phenolic compounds in this experiment were extracted from leaf and rhizome using different organic solvents. The antioxidant activity was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that total phenol from the leaf extracted by methanol, acetone, ethyl acetate, and hexane were 22.17 mg GAE/g, 15.89 mg GAE /g, 12.79 mg GAE /g, and 11.49 mg GAE/g respectively. The flavonoid content were 5.07 mg QE /g, 6.55 mg QE /g, 3.85 mg QE /g, and 2.23 mg QE /g respectively which were calculated base on quercetin standard. On the other hand, the total phenol and flavonoid content from karimenga rhizome extracted by each organic solvent were 240.32 mg GAE/g, 139.02 mg GAE/g, 99.63 mg GAE/g, 105.57 mg GAE/g, and 63.06 mg QE /g, 48.35 mg QE /g, 43.07 mg QE /g, 31.78 mg QE /g, respectively. The antioxidant activities of leaf and rhizome extracts expressed as free radical scavenging activity were in the range of 75 – 90 %. The main volatile compound detected by GC-MS was α- and β-asarone.Karimenga (Acorus calamus) is an underutilized plant that contains bioactive compounds such as phenolics, flavonoids, and terpenoids. The accumulation of phenolic compounds varies in each part of the plant; hence the phenolic compounds in this experiment were extracted from leaf and rhizome using different organic solvents. The antioxidant activity was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that total phenol from the leaf extracted by methanol, acetone, ethyl acetate, and hexane were 22.17 mg GAE/g, 15.89 mg GAE /g, 12.79 mg GAE /g, and 11.49 mg GAE/g respectively. The flavonoid content were 5.07 mg QE /g, 6.55 mg QE /g, 3.85 mg QE /g, and 2.23 mg QE /g respectively which were calculated base on quercetin standard. On the other hand, the total phenol and flavonoid content from karimenga rhizome extracted by each organic solvent were 240.32 mg GAE/g, 139.02 mg GAE/g, 99.63 mg GAE/g, 105.57 mg GAE/g, and 63.06 mg QE /g, 48.35 mg QE /g, 43.07 mg QE /g, 31.78 mg QE /g,...","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":"51 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"Antioxidant activity of karimenga (Acorus calamus)\",\"authors\":\"G. S. Djarkasi, Lana E. Lalujan, Erny J. N. Nurali, M. Sumual\",\"doi\":\"10.1063/1.5125555\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Karimenga (Acorus calamus) is an underutilized plant that contains bioactive compounds such as phenolics, flavonoids, and terpenoids. The accumulation of phenolic compounds varies in each part of the plant; hence the phenolic compounds in this experiment were extracted from leaf and rhizome using different organic solvents. The antioxidant activity was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that total phenol from the leaf extracted by methanol, acetone, ethyl acetate, and hexane were 22.17 mg GAE/g, 15.89 mg GAE /g, 12.79 mg GAE /g, and 11.49 mg GAE/g respectively. The flavonoid content were 5.07 mg QE /g, 6.55 mg QE /g, 3.85 mg QE /g, and 2.23 mg QE /g respectively which were calculated base on quercetin standard. On the other hand, the total phenol and flavonoid content from karimenga rhizome extracted by each organic solvent were 240.32 mg GAE/g, 139.02 mg GAE/g, 99.63 mg GAE/g, 105.57 mg GAE/g, and 63.06 mg QE /g, 48.35 mg QE /g, 43.07 mg QE /g, 31.78 mg QE /g, respectively. The antioxidant activities of leaf and rhizome extracts expressed as free radical scavenging activity were in the range of 75 – 90 %. The main volatile compound detected by GC-MS was α- and β-asarone.Karimenga (Acorus calamus) is an underutilized plant that contains bioactive compounds such as phenolics, flavonoids, and terpenoids. The accumulation of phenolic compounds varies in each part of the plant; hence the phenolic compounds in this experiment were extracted from leaf and rhizome using different organic solvents. The antioxidant activity was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that total phenol from the leaf extracted by methanol, acetone, ethyl acetate, and hexane were 22.17 mg GAE/g, 15.89 mg GAE /g, 12.79 mg GAE /g, and 11.49 mg GAE/g respectively. The flavonoid content were 5.07 mg QE /g, 6.55 mg QE /g, 3.85 mg QE /g, and 2.23 mg QE /g respectively which were calculated base on quercetin standard. On the other hand, the total phenol and flavonoid content from karimenga rhizome extracted by each organic solvent were 240.32 mg GAE/g, 139.02 mg GAE/g, 99.63 mg GAE/g, 105.57 mg GAE/g, and 63.06 mg QE /g, 48.35 mg QE /g, 43.07 mg QE /g, 31.78 mg QE /g,...\",\"PeriodicalId\":20581,\"journal\":{\"name\":\"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations\",\"volume\":\"51 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-09-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1063/1.5125555\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1063/1.5125555","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
摘要
菖蒲是一种未被充分利用的植物,它含有生物活性化合物,如酚类物质、类黄酮和萜类。酚类化合物的积累在植物的各个部位是不同的;因此,本实验采用不同的有机溶剂从叶和根茎中提取酚类化合物。采用2,2-二苯基-1-苦味酰肼(DPPH)法测定其抗氧化活性。结果表明,甲醇、丙酮、乙酸乙酯和己烷提取的总酚含量分别为22.17 mg GAE/g、15.89 mg GAE/g、12.79 mg GAE/g和11.49 mg GAE/g。按槲皮素标准计算,黄酮类含量分别为5.07 mg QE /g、6.55 mg QE /g、3.85 mg QE /g和2.23 mg QE /g。另一方面,各有机溶剂提取的根总酚和类黄酮含量分别为240.32 mg GAE/g、139.02 mg GAE/g、99.63 mg GAE/g、105.57 mg GAE/g和63.06 mg QE /g、48.35 mg QE /g、43.07 mg QE /g、31.78 mg QE /g。叶片和根茎提取物的抗氧化活性表现为清除自由基的活性在75% ~ 90%之间。GC-MS检测到的主要挥发性化合物为α-和β-细辛酮。菖蒲是一种未被充分利用的植物,它含有生物活性化合物,如酚类物质、类黄酮和萜类。酚类化合物的积累在植物的各个部位是不同的;因此,本实验采用不同的有机溶剂从叶和根茎中提取酚类化合物。采用2,2-二苯基-1-苦味酰肼(DPPH)法测定其抗氧化活性。结果表明,甲醇、丙酮、乙酸乙酯和己烷提取的总酚含量分别为22.17 mg GAE/g、15.89 mg GAE/g、12.79 mg GAE/g和11.49 mg GAE/g。按槲皮素标准计算,黄酮类含量分别为5.07 mg QE /g、6.55 mg QE /g、3.85 mg QE /g和2.23 mg QE /g。另一方面,各有机溶剂提取的木耳根总酚和总黄酮含量分别为240.32 mg GAE/g、139.02 mg GAE/g、99.63 mg GAE/g、105.57 mg GAE/g和63.06 mg QE /g、48.35 mg QE /g、43.07 mg QE /g、31.78 mg QE /g、…
Antioxidant activity of karimenga (Acorus calamus)
Karimenga (Acorus calamus) is an underutilized plant that contains bioactive compounds such as phenolics, flavonoids, and terpenoids. The accumulation of phenolic compounds varies in each part of the plant; hence the phenolic compounds in this experiment were extracted from leaf and rhizome using different organic solvents. The antioxidant activity was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that total phenol from the leaf extracted by methanol, acetone, ethyl acetate, and hexane were 22.17 mg GAE/g, 15.89 mg GAE /g, 12.79 mg GAE /g, and 11.49 mg GAE/g respectively. The flavonoid content were 5.07 mg QE /g, 6.55 mg QE /g, 3.85 mg QE /g, and 2.23 mg QE /g respectively which were calculated base on quercetin standard. On the other hand, the total phenol and flavonoid content from karimenga rhizome extracted by each organic solvent were 240.32 mg GAE/g, 139.02 mg GAE/g, 99.63 mg GAE/g, 105.57 mg GAE/g, and 63.06 mg QE /g, 48.35 mg QE /g, 43.07 mg QE /g, 31.78 mg QE /g, respectively. The antioxidant activities of leaf and rhizome extracts expressed as free radical scavenging activity were in the range of 75 – 90 %. The main volatile compound detected by GC-MS was α- and β-asarone.Karimenga (Acorus calamus) is an underutilized plant that contains bioactive compounds such as phenolics, flavonoids, and terpenoids. The accumulation of phenolic compounds varies in each part of the plant; hence the phenolic compounds in this experiment were extracted from leaf and rhizome using different organic solvents. The antioxidant activity was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that total phenol from the leaf extracted by methanol, acetone, ethyl acetate, and hexane were 22.17 mg GAE/g, 15.89 mg GAE /g, 12.79 mg GAE /g, and 11.49 mg GAE/g respectively. The flavonoid content were 5.07 mg QE /g, 6.55 mg QE /g, 3.85 mg QE /g, and 2.23 mg QE /g respectively which were calculated base on quercetin standard. On the other hand, the total phenol and flavonoid content from karimenga rhizome extracted by each organic solvent were 240.32 mg GAE/g, 139.02 mg GAE/g, 99.63 mg GAE/g, 105.57 mg GAE/g, and 63.06 mg QE /g, 48.35 mg QE /g, 43.07 mg QE /g, 31.78 mg QE /g,...