Tábatta Caroline Cerri França, Adelson Lemes da Silva Júnior, Leticia Rigo Tavares, L. C. Souza, F. D. Miranda, M. V. W. Caldeira
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引用次数: 0
摘要
本研究的目的是对双生副眼的ISSR引物进行研究,并进一步进行遗传鉴定。为此,在巴西阿雷格里州阿雷格里市联邦教育学院(Ciência e Tecnologia do Espírito Santo)附近的一个私人区域的森林碎片中收集了20个个体的叶子样本。为此,对43条ISSR引物进行了测试,筛选出10条引物,共提供91条条带,其中多态性条带57条,多态性为62.63%。引物的多态性信息含量(PIC = 0.27)表明引物的信息量中等,因此在该物种的研究中是有效的。然而,观察等位基因数(AO = 1.68)和有效等位基因数(AE = 1.41)以及Nei (H* = 0.23)和Shannon (I* = 0.35)的遗传多样性指数表明,该群体存在纯合位点,遗传多样性较低。另一方面,通过贝叶斯方法评估的遗传结构揭示了分布在所有样本个体中的三个遗传群的形成,再次推断了纯合性位点的发生。因此,相邻片段的连接和从其他来源获得的个体的建立可以增加群体的遗传多样性,减少近亲繁殖和遗传漂变可能造成的抑郁影响。
Prospection of ISSR primers and population genetic characterization of Paratecoma peroba (Record) Kuhlm.
The objective of this study is to prospect ISSR primers (Inter Simple Sequence Repeats) and, further genetic characterization in Paratecoma peroba. For this, leaf samples of 20 individuals were collected in a forest fragment, located in a private area, close to the Instituto Federal de Educação, Ciência e Tecnologia do Espírito Santo, in the city of Alegre, ES, Brazil. For this, 43 ISSR primers were tested, and 10 primers were selected, which provided a total of 91 bands, with 57 polymorphic bands, resulting in 62.63% polymorphism. The polymorphic information content (PIC = 0.27) indicated moderate informativeness of the primers and, therefore, they are efficient in studies with the species. However, the values found for genetic parameters such as the number of observed (AO = 1.68) and effective (AE = 1.41) alleles and, the genetic diversity indices of Nei (H* = 0.23) and Shannon (I* = 0.35) indicate the occurrence of homozygous loci and low genetic diversity in the population. On the other hand, the genetic structure evaluated by the Bayesian approach revealed the formation of three genetic groups distributed in all sampled individuals, inferring once again about the occurrence of loci in homozygosity. Therefore, the connection of neighbouring fragments and the establishment of individuals obtained from other sources could increase the genetic diversity of the population and reduce the possible effects of depression by inbreeding and genetic drift.
期刊介绍:
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.