森美兰州和雪兰莪州花蟹副溶血性弧菌的分离与鉴定

e-Academia Journal Pub Date : 2019-03-14 DOI:10.24191/E-AJ.V7ISI-TEMIC18.5399

Nur Rabiatul Adawiah B Mohammad Noor, S. Mutalib, N. A. Sani

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引用次数: 2

摘要

本研究对2014年3月至7月在森美兰州(n = 15)和雪兰莪州(n = 15)采集的30只花蟹(Portunus pelagicus)样本进行了副溶血性弧菌检测。从柠檬酸硫硫酸盐胆盐(TCBS)琼脂培养基中分离出193株弧菌。79株分离弧菌经生化检验鉴定为副溶血性弧菌。在检测调节基因toxR的副溶血性弧菌鉴定中，79株分离株中有51株(64.6%)含有toxR基因，未分离株含有tdh和trh毒力基因。该基因产生分子量为368bp的扩增子。所有分离的副溶血性弧菌tdh和trh基因均为阴性。关键词:副溶血性弧菌，花蟹，toxR, trh, tdh

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ISOLATION AND IDENTIFICATION OF Vibrio parahaemolyticus FROM FLOWER CRAB (Portunus pelagicus) IN NEGERI SEMBILAN AND SELANGOR

This study was conducted to determine the presence of Vibrio parahaemolyticus on 30 samples of flower crab (Portunus pelagicus) collected from Negeri Sembilan (n = 15) and Selangor (n = 15) from March to July 2014. A total of 193 isolates of Vibrio sp. were isolated from the thiosulphate citrate bile-salt (TCBS) agar medium. Of 79 Vibrio isolates were identified as V. parahaemolyticus through biochemical testing methods. In V. parahaemolyticus identification by detection of regulatory gene toxR, 51 (64.6%) of 79 isolates contained toxR gene while none of the isolates contained virulence gene of tdh dan trh. by targeting toxR gene which produced amplicons of 368 bp molecular weight. All V. parahaemolyticus isolates were negative for tdh and trh genes. Keywords: Vibrio parahaemolyicus, flower crab, toxR, trh, tdh

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