ANALYSIS OF FRACTION EFFICIENCY OF GALLERIA MELLONELLA L. WASTE PRODUCTS UNDER EXPERIMENTAL STRESS

The aim of the research was to study the stress level in terms of parametres of peripheral blood and histology of the internal organs of mice when using various fractions of the extract based on waste products of Galleria mellonella L. For the experiment, three groups of laboratory mice were formed according to the principle of analogue pairs (n=5). An extract of the light fraction of waste products and a 40% alcohol solution (control) were administered transdermally through the skin of the tail of mice every other day for 30 days at a dose of 5 μl. Method of experimental modeling of general hyperthermia was chosen for the experiment. Statistically significant differences in leukocyte formula indexes of mice in the experimental group of light fraction of waste products of G. mellonella larvae were found compared with the control. The sum of young and rod-shaped neutrophils in the control group is greater than the same parametre in the experimental group, which indicates stimulation of neutrophil production in the control group of mice, this is usually associated with pathological processes in the body. The obtained data show reactivity and mobilization of body defenses of mice under the influence of a light fraction of the waste products of G. mellonella larvae, and therefore, stress resistance increases. A decrease of fascicular zone occurs in case of transdermal administration of light fraction solution, which can lead to a decrease of secretion of glucocorticoids. The obtained results are the basis for further laboratory and theoretical studies of the light fraction as an adaptogenic agent in animal husbandry in order to improve productive qualities of animals.