儿茶酚胺及其代谢物的检测与标准化

Q4 Health Professions

中华检验医学杂志 Pub Date : 2020-03-11 DOI:10.3760/CMA.J.ISSN.1009-9158.2020.03.024

Qingxiang Liu, Weiyan Zhou, Chuanbao Zhang

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引用次数: 0

摘要

儿茶酚胺包括多巴胺、去甲肾上腺素和肾上腺素。主要代谢产物为3-甲氧基酪胺、去甲肾上腺素、去甲肾上腺素、同香草酸和香草酸。儿茶酚胺及其代谢物的检测是诊断嗜铬细胞瘤、副神经节瘤、神经母细胞瘤等神经嵴源性神经内分泌肿瘤的基础。液相色谱串联质谱法以其高灵敏度和高特异性在儿茶酚胺及其代谢物的检测中得到了广泛的应用。但不同实验室的液相色谱串联质谱分析结果差异较大，缺乏可比性。儿茶酚胺及其代谢物在血浆和尿液中易氧化降解，存在许多干扰，且在血浆和尿液样本中浓度低，因此准确测定儿茶酚胺及其代谢物目前是临床检测领域的一个挑战。关键词:儿茶酚胺;神经内分泌肿瘤;色谱法、液体;串联质谱法

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Detection and standardization of catecholamines and their metabolites

Catecholamines include dopamine, norepinephrine and epinephrine. The main metabolites are 3-methoxytyramine, normetanephrine, metanephrine,homovanillic acid and vanillylmandelic acid. Detection of catecholamines and their metabolites is the cornerstone for the diagnosis of neuroendocrine tumors derived from neural crest such as pheochromocytoma, paraganglioma and neuroblastoma. Liquid chromatography tandem mass spectrometry has been widely used in the detection of catecholamines and their metabolites due to its high sensitivity and high specificity. However, the results of liquid chromatography tandem mass spectrometry in different laboratories are quite different and lack comparability. Accurate determination of catecholamines and their mtabolites in plasma and urine is currently a challenge in the field of clinical detectionbecause of their susceptibility to oxidative degradation, presence of numerous interferences and low concentration in plasma and urine samples. Key words: Catecholamines; Neuroendocrine tumor; Chromatography, liquid; Tandem mass spectrometry

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来源期刊

中华检验医学杂志 Health Professions-Medical Laboratory Technology

CiteScore

0.40

自引率

0.00%

发文量

8037

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