大肠杆菌对Balb/C大鼠早产模型的影响

IF 0.2 Q4 PHARMACOLOGY & PHARMACY
Suheni Ninik Hariyati, D. Santosaningsih, Arief Satria Hardika, Fikhy rizky Hapsari, Nirwan Nugraha, Wildan Aulia Firdaus
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摘要

引言:印度尼西亚的早产患病率估计为7-14%,即每年约有459,200-900,000名婴儿。感染的机制是早产的最大原因。经常引起感染的细菌是大肠杆菌。这项研究的目的是观察给予大肠杆菌产生过早小鼠模型的效果。材料与方法:本研究采用真正的实验方法,仅采用测试后对照组设计方法。独立变量为早产和宫颈组织培养、羊膜和胎盘。该研究的因变量是给子宫颈注射大肠杆菌109 CFU。这项研究将用比较试验进行检验。结果:治疗组给予大肠杆菌分娩,最快时间为38小时22分钟,最长时间为42小时12分钟。大肠杆菌菌落在伊红亚甲基蓝(EMB)培养基上,呈现绿色发光图像。治疗组Balb/C小鼠宫颈培养物、羊膜和胎盘均有大肠杆菌菌落生长。大肠杆菌浓度为109 CFU/ml的Balb/C小鼠颈间质中性粒细胞浸润和水肿更为严重。结论:大肠杆菌缩短了Balb/C妊娠小鼠的分娩时间,增加了细菌载量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Effect of Establishing Escherichia coli on Balb/C Rats as A Model of Preterm Labor
Introduction: The prevalence of preterm labor in Indonesia is estimated at 7-14%, which is around 459,200-900,000 babies per year. The mechanism of infection is the biggest cause of preterm labor. The bacteria that often causes infection is Escherichia coli. This study aims to see the effect of giving Escherichia coli to produce premature models of mice. Material and Methods: This study used a true experimental method with a post-test only control group design approach. The independent variables were preterm labor & cervical tissue culture, amniotic membranes, and placenta. The dependent variable of the study was the administration of Escherichia coli 109 CFU to the cervix. This research will be tested with a comparative test. Results: The treatment group were given Escherichia coli experienced parturition with the fastest time of 38 hours 22 minutes and the longest time of 42 hours 12 minutes. Escherichia coli colonies on Eosin Methylene Blue (EMB) culture media with a glowing green image was obtained. Cervical culture, amniotic membranes and placenta from the treatment group of Balb/C mice showed the growth of Escherichia coli colonies. Neutrophil infiltration and edema in the cervical stroma was found to be more severe in Balb/C mice that received treatment with Escherichia coli 109 CFU/ml. Conclusion: Escherichia coli shortens the time of delivery and increases the bacterial load in pregnant Balb/C mice.
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